Pan Lingmei, Jaroenporn Sukanya, Yamamoto Tatsuya, Nagaoka Kentaro, Azumano Isao, Onda Masaaki, Watanabe Gen, Taya Kazuyoshi
Department of Basic Veterinary Science, The United School of Veterinary Sciences Gifu University 501-1193 Gifu Japan.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture Tokyo University of Agriculture and Technology 183-8509 Tokyo Fuchu Japan.
Reprod Med Biol. 2011 Dec 2;11(2):101-104. doi: 10.1007/s12522-011-0113-6. eCollection 2012 Apr.
The effect of pantothenic acid (PaA) supplementation on adrenal secretion of corticosterone and progesterone in female rats was investigated.
An in-vitro primary adrenal cell culture system was used. Pregnant rats were given 0.03% PaA in their drinking water throughout pregnancy and the period of lactation. In the first experiment, after weaning, female rats continued to receive 0.03% PaA treatment until 10 weeks of age. The animals were then decapitated and adrenal cells were cultured in the absence or presence of rat adrenocorticotropic hormone (ACTH) for 4 h. In the second experiment, adrenal cells from lactating rats on day 5 of lactation were cultured in the absence or presence of rat ACTH for 4 h.
The effect of ACTH at 10 m on corticosterone and progesterone release was greater for PaA-treated cyclic rats than for control cyclic rats. The effect of ACTH at 10 m on corticosterone release was greater for PaA-treated lactating rats than for control lactating rats. Circulating ACTH and corticosterone levels in PaA-treated and control cyclic and lactating rats were no different.
These results indicate that PaA supplementation induced hyperresponsiveness to ACTH stimulation in cyclic and lactating female rats. These results clearly demonstrated that PaA is an essential factor in adrenal steroidogenesis of female rats.
研究补充泛酸（PaA）对雌性大鼠肾上腺皮质酮和孕酮分泌的影响。
采用体外原代肾上腺细胞培养系统。妊娠大鼠在整个孕期和哺乳期的饮水中添加0.03%的PaA。在第一个实验中，断奶后，雌性大鼠继续接受0.03%的PaA处理，直至10周龄。然后将动物断头，肾上腺细胞在有无大鼠促肾上腺皮质激素（ACTH）的情况下培养4小时。在第二个实验中，将哺乳期第5天的哺乳大鼠的肾上腺细胞在有无大鼠ACTH的情况下培养4小时。
对于经PaA处理的周期性大鼠，10 m的ACTH对皮质酮和孕酮释放的影响比对对照周期性大鼠的影响更大。对于经PaA处理的哺乳大鼠，10 m的ACTH对皮质酮释放的影响比对对照哺乳大鼠的影响更大。经PaA处理的和对照的周期性及哺乳大鼠的循环ACTH和皮质酮水平没有差异。
这些结果表明，补充PaA可诱导周期性和哺乳雌性大鼠对ACTH刺激产生高反应性。这些结果清楚地表明，PaA是雌性大鼠肾上腺类固醇生成的一个必需因素。
