Effects of pantothenic acid supplement on secretion of steroids by the adrenal cortex in female rats.
作者信息
Pan Lingmei, Jaroenporn Sukanya, Yamamoto Tatsuya, Nagaoka Kentaro, Azumano Isao, Onda Masaaki, Watanabe Gen, Taya Kazuyoshi
机构信息
Department of Basic Veterinary Science, The United School of Veterinary Sciences Gifu University 501-1193 Gifu Japan.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture Tokyo University of Agriculture and Technology 183-8509 Tokyo Fuchu Japan.
出版信息
Reprod Med Biol. 2011 Dec 2;11(2):101-104. doi: 10.1007/s12522-011-0113-6. eCollection 2012 Apr.
PURPOSE
The effect of pantothenic acid (PaA) supplementation on adrenal secretion of corticosterone and progesterone in female rats was investigated.
METHODS
An in-vitro primary adrenal cell culture system was used. Pregnant rats were given 0.03% PaA in their drinking water throughout pregnancy and the period of lactation. In the first experiment, after weaning, female rats continued to receive 0.03% PaA treatment until 10 weeks of age. The animals were then decapitated and adrenal cells were cultured in the absence or presence of rat adrenocorticotropic hormone (ACTH) for 4 h. In the second experiment, adrenal cells from lactating rats on day 5 of lactation were cultured in the absence or presence of rat ACTH for 4 h.
RESULTS
The effect of ACTH at 10 m on corticosterone and progesterone release was greater for PaA-treated cyclic rats than for control cyclic rats. The effect of ACTH at 10 m on corticosterone release was greater for PaA-treated lactating rats than for control lactating rats. Circulating ACTH and corticosterone levels in PaA-treated and control cyclic and lactating rats were no different.
CONCLUSIONS
These results indicate that PaA supplementation induced hyperresponsiveness to ACTH stimulation in cyclic and lactating female rats. These results clearly demonstrated that PaA is an essential factor in adrenal steroidogenesis of female rats.
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