Teramura Takeshi, Frampton John
Institute of Advanced Clinical Medicine Kinki University Faculty of Medicine 377-2 Osaka-sayama Osaka Japan.
Department of Obstetrics and Gynecology Mie University Faculty of Medicine Tsu Mie Japan.
Reprod Med Biol. 2012 Dec 14;12(2):39-46. doi: 10.1007/s12522-012-0141-x. eCollection 2013 Apr.
Despite recent advances in reproductive medicine, there are still no effective treatments for severe infertility caused by congenital absence of germ cells or gonadotoxic treatments during prepubertal childhood. However, the development of technologies for germ cell formation from stem cells in vitro, induction of pluripotency from somatic cells, and production of patient-specific pluripotent stem cells may provide new solutions for treating these severe fertility problems. It may be possible to produce germ cells in vitro from our own somatic cells that can be used to restore fertility. In addition, these technologies may also bring about novel therapies by helping to elucidate the mechanisms of human germ cell development. In this review, we describe the current approaches for obtaining germ cells from pluripotent stem cells, and provide basic information about induction of pluripotency and germ cell development.
尽管生殖医学最近取得了进展，但对于青春期前儿童期因先天性生殖细胞缺失或性腺毒性治疗导致的严重不孕症，仍然没有有效的治疗方法。然而，体外由干细胞形成生殖细胞、体细胞诱导多能性以及产生患者特异性多能干细胞等技术的发展，可能为治疗这些严重的生育问题提供新的解决方案。有可能从我们自己的体细胞中体外产生可用于恢复生育能力的生殖细胞。此外，这些技术还可能通过帮助阐明人类生殖细胞发育机制带来新的疗法。在本综述中，我们描述了从多能干细胞获得生殖细胞的当前方法，并提供了有关多能性诱导和生殖细胞发育的基本信息。
