Aoki Kazuo, Kihaile Paul E, Misumi Junichi, Pei Wei, Kudo Masanobu
Division of Preventive Medicine, Department of Human Environmental and Social Medicine, Faculty of Medicine, Oita University, Hasama, Oita, Japan.
Reprod Med Biol. 2004 May 20;3(2):59-62. doi: 10.1111/j.1447-0578.2004.00053.x. eCollection 2004 Jun.
2,5-Hexanedione is an industrial solvent which causes peripheral neuropathy. In the current study, the effect of 2,5-hexanedione on testicular histology of adult rats as well as sperm concentration, motility, and morphology were studied by administering 100 mg, 200 mg, 400 mg/kg per day for 12 weeks. No sperm motility was observed in the 200 mg and 400 mg/kg per day treatment groups and significantly reduced motility was observed in the 100 mg/kg per day group. The morphology were also significantly reduced in the 200 mg and 400 mg/kg per day groups compared to the control group, but the sperm concentration was significantly reduced only in the 400 mg/kg per day group. Histological examination of the testes in the 400 mg/kg per day group revealed that two-thirds of the testes had Sertoli cell only syndrome, whereas in the 200 mg/kg per day group half of the testes showed maturation arrest and sperm as well as spermatids were observed in 83% of the testes. In conclusion, we have shown that 2,5-hexanedione severely affected sperm motility even at low doses, whereas high doses adversely affected all the sperm parameters as well as causing testicular injury. (Reprod Med Biol 2004; : 59-62).
2,5 - 己二酮是一种可导致周围神经病变的工业溶剂。在本研究中，通过每天给予成年大鼠100毫克、200毫克、400毫克/千克的2,5 - 己二酮，持续12周，研究其对成年大鼠睾丸组织学以及精子浓度、活力和形态的影响。在每天200毫克和400毫克/千克的治疗组中未观察到精子活力，在每天100毫克/千克的组中观察到活力显著降低。与对照组相比，每天200毫克和400毫克/千克组的精子形态也显著降低，但仅在每天400毫克/千克组精子浓度显著降低。对每天400毫克/千克组的睾丸进行组织学检查发现，三分之二的睾丸出现唯支持细胞综合征，而在每天200毫克/千克组中，一半的睾丸显示成熟停滞，83%的睾丸中观察到精子和精子细胞。总之，我们已经表明，2,5 - 己二酮即使在低剂量下也会严重影响精子活力，而高剂量会对所有精子参数产生不利影响，并导致睾丸损伤。（《生殖医学与生物学》2004年；：59 - 62）
