Farag A T, Eweidah M H, El-Okazy A M
Department of Pesticide Chemistry, Faculty of Agriculture (El-Shatby), University of Alexandria, Alexandria, Egypt.
Reprod Toxicol. 2000 Sep-Oct;14(5):457-62. doi: 10.1016/s0890-6238(00)00094-0.
The reproductive toxicity of the insecticide acephate was studied in male mice. Adult male mice were treated by gavage with acephate at doses of 0, 7, 14, and 28 mg/kg/day for 4 weeks before mating with untreated females. Signs of cholinergic effects were observed in the 28 mg/kg/day group. Brain and skeletal muscle acetylcholinesterase activity was inhibited only in this group. Acephate treatment was associated with a decreased number of implantations and live fetuses, and an increased number of early resorptions at 28 mg/kg/day. The percent morphologically normal spermatozoa was unaffected in all dose groups; however, sperm motility and count were decreased in the 14 and 28 mg/kg/day groups compared to the control. Histologic examination of brain did not reveal any abnormalities. Dose related histologic changes, including degeneration of muscle fibers, were observed in the muscles of male mice treated with any of the doses of acephate. The current study demonstrated adverse effects of male acephate exposure on pregnancy outcome with effects on sperm parameters at 14 and 28 mg/kg/day.
在雄性小鼠中研究了杀虫剂乙酰甲胺磷的生殖毒性。成年雄性小鼠在与未处理的雌性小鼠交配前4周，通过灌胃给予剂量为0、7、14和28 mg/kg/天的乙酰甲胺磷。在28 mg/kg/天组中观察到胆碱能效应的迹象。仅在该组中脑和骨骼肌乙酰胆碱酯酶活性受到抑制。在28 mg/kg/天剂量下，乙酰甲胺磷处理与着床和活胎数量减少以及早期吸收数量增加有关。所有剂量组中形态正常精子的百分比未受影响；然而，与对照组相比，14和28 mg/kg/天组的精子活力和计数降低。脑的组织学检查未发现任何异常。在用任何剂量的乙酰甲胺磷处理的雄性小鼠肌肉中观察到与剂量相关的组织学变化，包括肌纤维变性。当前研究表明，雄性小鼠暴露于乙酰甲胺磷对妊娠结局有不良影响，在14和28 mg/kg/天剂量下对精子参数有影响。
