Yano Kohji, Kubo Toshiko, Ôhashi Ikuko, Yano Chieko
IVF Center, Yano Maternity Clinic, Ehime, Japan.
Reprod Med Biol. 2006 Aug 9;5(3):221-226. doi: 10.1111/j.1447-0578.2006.00145.x. eCollection 2006 Sep.
We assessed the effectiveness of assisted hatching using a 1.48-µm diode laser in human embryos, comparing zona opening and zona thinning techniques. A total of 56 day 3 embryos were assigned randomly to a zona opening group, a zona thinning group, or a control group. We then carried out assisted hatching using the OCTAX Laser Shot system (MTG Medical Technology, Altdorf, Germany) in the first two groups. In the zona opening group, the inner membrane of the zona pellucida was broken to create a full-thickness opening. In the zona thinning group, the inner membrane of the zona pellucida was not breached. After the laser procedure, embryos were cultured to the hatched blastocyst stage. Blastocyst development rates did not differ significantly between the three groups. In the zona opening group, blastocysts were significantly more likely to hatch than those in the control group ( ≤ 0.05) and no arrested hatching of blastocysts was observed. Assisted hatching using a 1.48-µm diode laser in the zona opening technique increases the likelihood of blastocyst hatching in human embryos and does not adversely effect subsequent embryo development. (Reprod Med Biol 2006; : 221-226).
我们评估了使用1.48微米二极管激光对人类胚胎进行辅助孵化的效果，比较了透明带开口和透明带变薄技术。总共56个第3天的胚胎被随机分配到透明带开口组、透明带变薄组或对照组。然后我们在前两组中使用OCTAX激光射击系统（MTG医疗技术公司，德国阿尔特多夫）进行辅助孵化。在透明带开口组中，打破透明带的内膜以形成全层开口。在透明带变薄组中，透明带的内膜未被破坏。激光操作后，将胚胎培养至孵化囊胚阶段。三组之间的囊胚发育率没有显著差异。在透明带开口组中，囊胚孵化的可能性明显高于对照组（≤0.05），并且未观察到囊胚孵化停滞。在透明带开口技术中使用1.48微米二极管激光进行辅助孵化可增加人类胚胎中囊胚孵化的可能性，并且不会对后续胚胎发育产生不利影响。（《生殖医学与生物学》2006年；：221 - 226）
