Yamanaka Ken-Ichi, Aono Nobuya, Yoshida Hiroaki, Sato Eimei
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University and.
Center for Advanced Reproductive Endocrinology, Yosida Lady's Clinic, Sendai, Japan.
Reprod Med Biol. 2007 May 14;6(2):61-68. doi: 10.1111/j.1447-0578.2007.00167.x. eCollection 2007 Jun.
Cryopreservation, maturation, fertilization and culture can be applied to various processes across a wide range of species, that is, for the breeding and reproduction of farm animals, preservation of genetic variants in laboratory animals, and the conservation of wild species. In particular, the storage of oocytes by cryopreservation and IVM following cryopreservation, might become effective alternative assisted reproduction treatments for infertile patients. For example, in a clinical context, these techniques might be important for patients who are at risk of losing their ovarian function because of extirpative therapy, chemotherapy or radiation. Thus, it is important for assisted reproductive technology to improve IVM and cryopreservation techniques. In the present review, we introduce our recent studies on vitrification and IVM of germinal vesicle stage oocytes in animals.
冷冻保存、成熟、受精和培养可应用于广泛物种的各种过程，即用于农场动物的繁殖和育种、实验动物遗传变异体的保存以及野生物种的保护。特别是，通过冷冻保存和冷冻保存后的体外成熟（IVM）来储存卵母细胞，可能成为不育患者有效的辅助生殖替代治疗方法。例如，在临床环境中，这些技术对于因切除疗法、化疗或放疗而有失去卵巢功能风险的患者可能很重要。因此，辅助生殖技术改进体外成熟和冷冻保存技术非常重要。在本综述中，我们介绍了我们最近关于动物生发泡期卵母细胞玻璃化和体外成熟的研究。
