冷冻保存卵巢中小鼠卵母细胞-颗粒细胞复合体的生长与成熟及幼崽出生的实现
growth and maturation of mouse oocyte-granulosa cell complex from cryopreserved ovaries and achievement of pup birth.
作者信息
Hasegawa Akiko, Koyama Koji
机构信息
Laboratory of Developmental Biology and Reproduction, Institute for Advanced Medical Sciences and.
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.
出版信息
Reprod Med Biol. 2007 May 14;6(2):77-83. doi: 10.1111/j.1447-0578.2007.00169.x. eCollection 2007 Jun.
Ovarian tissue banking is a feasible strategy for fertility preservation for young women after cancer treatments. Ovarian tissue, after thawing, is used for several options; orthotopic grafting (normal site), autologous heterotopic grafting and collection of ovarian follicles for culture. Recent reports of live birth encouraged clinicians and researchers to apply this technology to premature ovarian failure (POF) resulting from strong cancer therapy. Grafting, however, carries a risk of malignant cell recurrence. For safety, development of a culture method is necessary but optimum culturing conditions for less-developed follicles abundant in the ovary are not well known. In the present article, the current status of ovarian tissue cryopreservation, and oocyte growth and maturation from the preserved ovaries are reviewed.
卵巢组织冻存是癌症治疗后年轻女性生育力保存的一种可行策略。解冻后的卵巢组织有多种用途，包括原位移植（正常位置）、自体异位移植以及收集卵巢卵泡进行培养。近期关于活产的报道促使临床医生和研究人员将这项技术应用于因强力癌症治疗导致的卵巢早衰（POF）。然而，移植存在恶性细胞复发的风险。为了安全起见，开发一种培养方法是必要的，但卵巢中大量存在的发育不成熟卵泡的最佳培养条件尚不清楚。在本文中，对卵巢组织冷冻保存的现状以及从保存的卵巢中获取卵母细胞的生长和成熟情况进行了综述。
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