Kuji Naoaki, Mizusawa Yuri, Naganishi Miwa, Hamatani Toshio, Iwata Sokichi, Yoshimura Yasunori
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Reprod Med Biol. 2007 Aug 6;6(3):151-156. doi: 10.1111/j.1447-0578.2007.00178.x. eCollection 2007 Sep.
As HIV infection is becoming a controllable chronic infection after the introduction of the highly active antiretroviral therapy (HAART), patients with this infection are now able to make plans for the future. Numerous serodiscordant (HIV-positive men and HIV-negative women) couples seek medical assistance to have a child with minimum risk to the HIV-negative female partner and the child using processed (washed) sperm. HIV-1 elimination from the semen using density gradient centrifugation and the swim-up procedure, which is a standard processing procedure, depends on both sperm motility and the physical properties of the virus-containing particles. To establish a simple method for recovering sperm from poor quality semen, we developed the tilted-tube rotation method to generate a continuous density gradient, and using this continuous density gradient we have succeeded in recovering motile sperm, even from the semen of seropositive males with severe male factors. This review aims to summarize the recent progress in the underlying principles and technical aspects of semen processing for elimination of HIV-1. (Reprod Med Biol 2007; : 151-156).
随着高效抗逆转录病毒疗法（HAART）的引入，HIV感染正逐渐成为一种可控的慢性感染，感染这种病毒的患者现在能够为未来制定计划。许多血清学不一致的夫妇（HIV阳性男性和HIV阴性女性）寻求医疗帮助，以便通过使用经过处理（洗涤）的精子，让HIV阴性女性伴侣和孩子面临最小风险地生育一个孩子。使用密度梯度离心和上游法从精液中去除HIV-1，这是一种标准的处理程序，它既取决于精子活力，也取决于含病毒颗粒的物理特性。为了建立一种从质量差的精液中回收精子的简单方法，我们开发了倾斜管旋转法以产生连续密度梯度，并且利用这种连续密度梯度，我们成功地从精液质量差的血清阳性男性精液中回收了活动精子。本综述旨在总结精液处理以消除HIV-1的基本原理和技术方面的最新进展。（《生殖医学与生物学》2007年；：151 - 156）
