Inhibition of apoptosis by ascorbic and dehydroascorbic acids in egg extracts.

PURPOSE

The viability of mammalian eggs after ovulation is reported to be improved by the presence of ascorbic acid in the culture medium. However, the pro-survival mechanisms of ascorbic acid are poorly understood. The molecular pathways of apoptosis are evolutionarily conserved among animal species, and eggs are technically and ethically more suitable for biochemical analyses than mammalian eggs. We used egg cytoplasmic extracts to examine the direct intracellular effects of ascorbic acid.

METHODS

Incubation of egg extracts for more than 4 h induces the spontaneous release of cytochrome from mitochondria. This event triggers the activation of caspases, cleavage of substrate proteins, and execution of apoptosis. Multiple signal transduction pathways including proteolysis and protein phosphorylation are also involved in this process. We examined whether any of these events might be inhibited by the addition of ascorbic acid.

RESULTS

Ascorbic acid showed no effect against cytochrome release, but prevented caspase activation and substrate cleavage. Ascorbic acid also blocked the proteolysis of apoptosis inhibitor proteins and the dephosphorylation of p42 MAP kinase. However, dehydroascorbic acid (oxidized form of ascorbic acid) and acetate (unrelated acid) were equally effective, indicating that these effects were primarily due to their acidity. In addition, dehydroascorbic acid inhibited caspase activities directly in vitro.

CONCLUSIONS

The anti-apoptotic effect of ascorbic acid in egg extracts is mainly due to cytoplasmic acidification rather than its intracellular antioxidant activity. Instead, oxidative conversion of ascorbic acid into dehydroascorbic acid may inhibit apoptosis through the inhibition of caspases.