谷胱甘肽对显微授精后大鼠胚胎发育的影响。

Effect of glutathione on the development of rat embryos following microinsemination.

作者信息

Yuzawa K, Sawada T, Hossain M S, Takagi Y, Hamano K, Tsujii H

机构信息

Laboratory of Animal Biotechnology, Faculty of Agriculture Shinshu University Minamiminowa-mura 399-4598 Nagano Japan.

Nagoya Reproduction Center The Sawada Women's Clinic Chikusaku Nagoya Aichi Japan.

出版信息

Reprod Med Biol. 2008 Dec 13;8(1):11-17. doi: 10.1007/s12522-008-0002-9. eCollection 2009 Mar.

DOI:10.1007/s12522-008-0002-9

PMID:29699302

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5906869/

Abstract

PURPOSE

The present study was carried out to evaluate the role of glutathione on rat embryo developmental potential after ICSI. We observed the effects of glutathione on the development of non-treated rat embryos, ICSI embryos and embryos with sham injection treatment. The development of glutathione-microinjected embryos was also observed.

METHODS

Oocytes and fertilized embryos were obtained from superovulated Wistar-Imamichi rats and cultured in mR1ECM medium. Oocytes and embryos were then allowed to develop to assess the effect of glutathione on the development rate in intact embryos, micro-injected embryos and ICSI embryos.

RESULTS

(1) In the intact embryo, the proportion of blastocyst stage development increased when 0.01 mM GSH was added to the medium compared to the control. (2) Microinjection of glutathione (GSSG, GSH) into the embryo increased development at each stage, and the addition of 0.2 nM GSSG or GSH significantly increased blastocyst development, in comparison to that of the control ( < 0.05). (3) Compared to the control, all the GSSG and GSH concentrations improved damaged blastocyst development, where 0.01 mM GSH improved significantly ( < 0.05). (4) The addition of glutathione in the medium increased the rate of blastocyst development after ICSI. A significantly higher number of TE and total cells were obtained in the micro-injected embryo with both of the 0.02 mM GSSG and GSH treatments ( < 0.05).

CONCLUSIONS

The addition of glutathione into the culture media can improve early embryo development and is capable of repairing the damage of ICSI rat embryos.

摘要

目的

本研究旨在评估谷胱甘肽对卵胞浆内单精子注射（ICSI）后大鼠胚胎发育潜能的作用。我们观察了谷胱甘肽对未处理的大鼠胚胎、ICSI胚胎和假注射处理胚胎发育的影响。还观察了显微注射谷胱甘肽的胚胎的发育情况。

方法

从超排卵的Wistar-Imamichi大鼠获取卵母细胞和受精卵，并在mR1ECM培养基中培养。然后让卵母细胞和胚胎发育，以评估谷胱甘肽对完整胚胎、显微注射胚胎和ICSI胚胎发育率的影响。

结果

（1）在完整胚胎中，与对照组相比，向培养基中添加0.01 mM谷胱甘肽时，囊胚期发育比例增加。（2）向胚胎显微注射谷胱甘肽（氧化型谷胱甘肽、还原型谷胱甘肽）可提高各阶段的发育率，与对照组相比，添加0.2 nM氧化型谷胱甘肽或还原型谷胱甘肽可显著提高囊胚发育率（P<0.05）。（3）与对照组相比，所有氧化型谷胱甘肽和还原型谷胱甘肽浓度均改善了受损囊胚的发育，其中0.01 mM还原型谷胱甘肽改善显著（P<0.05）。（4）培养基中添加谷胱甘肽可提高ICSI后囊胚发育率。用0.02 mM氧化型谷胱甘肽和还原型谷胱甘肽处理的显微注射胚胎中，滋养外胚层细胞和总细胞数量均显著增加（P<0.05）。