牛睾丸提取物对小鼠睾丸17β-羟类固醇脱氢酶3和肝脏细胞色素P450 1A2酶的下调作用。
Down-regulation of murine testicular 17β-HSD3 and hepatic CYP1A2 enzymes by a bovine testes extract.
作者信息
Chatuphonprasert Waranya, Thadsri Tawiphark, Jarukamjorn Kanokwan
机构信息
Academic Office for Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences Khon Kaen University Mitrapaab Road 40002 Khon Kaen Thailand.
出版信息
Reprod Med Biol. 2009 Nov 12;9(1):51-56. doi: 10.1007/s12522-009-0040-y. eCollection 2010 Mar.
PURPOSE
We investigated the effects of a bovine testes extract (BTE), which was developed as an alternative product for andropausal men, on expression of testicular enzymes responsible for sex hormone synthesis genes and a carcinogen activation related gene.
METHODS
Expression of testicular CYP1A2, CYP11A1, CYP17, 3β-HSD, and 17β-HSD3 mRNAs as well as hepatic CYP1A2 mRNA were semi-quantitatively determined by RT-PCR. In addition, expression of hepatic CYP1A2 protein and methoxyresorufin -demethylase activity were carried out.
RESULTS
Bovine testes extract did not alter the testicular expression of CYP11A1, CYP17, and 3β-HSD mRNAs, while that of CYP11A1 was significantly down-regulated by testosterone. Interestingly, administration of BTE for 3 weeks significantly suppressed testicular 17β-HSD3 and hepatic CYP1A2 mRNA. Correspondingly, methoxyresorufin -demethylase (MROD) activity and expression of hepatic CYP1A2 protein were significantly decreased.
CONCLUSIONS
These findings strongly suggested considering risks versus benefits and raised concerns regarding the use of BTE as an alternative medication or health supplement in andropausal men due to its potential for suppressing expression of both 17β-HSD3 and CYP1A2 mRNAs, testicular enzymes responsible for sex hormone gene synthesis.
目的
我们研究了一种作为男性更年期替代产品开发的牛睾丸提取物（BTE）对负责性激素合成基因和致癌物激活相关基因的睾丸酶表达的影响。
方法
通过逆转录聚合酶链反应（RT-PCR）半定量测定睾丸中细胞色素P450 1A2（CYP1A2）、细胞色素P450 11A1（CYP11A1）、细胞色素P450 17（CYP17）、3β-羟基类固醇脱氢酶（3β-HSD）和17β-羟基类固醇脱氢酶3（17β-HSD3）mRNA以及肝脏中CYP1A2 mRNA的表达。此外，还进行了肝脏CYP1A2蛋白表达和甲氧基试卤灵-O-脱甲基酶活性测定。
结果
牛睾丸提取物未改变睾丸中CYP11A1、CYP17和3β-HSD mRNA的表达，而睾酮显著下调了CYP11A1的表达。有趣的是，给予BTE 3周显著抑制了睾丸17β-HSD3和肝脏CYP1A2 mRNA的表达。相应地，甲氧基试卤灵-O-脱甲基酶（MROD）活性和肝脏CYP1A2蛋白表达显著降低。
结论
这些发现强烈提示应权衡风险与益处，并且由于BTE有抑制负责性激素基因合成的睾丸酶17β-HSD3和CYP1A2 mRNA表达的可能性，因此对于在男性更年期男性中使用BTE作为替代药物或健康补充剂引发了担忧。
Zotero 插件