Department of Applied Science, National Taitung University, Taitung, Taiwan.
Department of Environment Engineering, Kun Shan University, Tainan, Taiwan.
Food Chem. 2018 Sep 15;260:61-65. doi: 10.1016/j.foodchem.2018.03.149. Epub 2018 Apr 2.
A simple approach to fabricate a highly selective and sensitive dimethoate probe was developed based on Rhodamine B (RB)-functionalized gold nanoparticles (AuNPs). The quenching of RB fluorescence in the presence of AuNPs in the solution, mediated by fluorescence resonance energy transfer, was observed. In the presence of dimethoate, the fluorescence intensity of the RB-AuNP solution is gradually recovered when dimethoate molecules displace RB molecules on the surface of the AuNPs, which significantly increased the fluorescence intensity of RB. Fluorescence is proportional to the dimethoate concentration in the range of 0.005-1.0 ppm (R = 0.989), and the LOD was 0.004 ppm. The recoveries of dimethoate in water and fruit samples were 86-116% with a good RSD (< 9.3%). Because of its high sensitivity, excellent selectivity, and convenient fabrication process, this method is a promising candidate for dimethoate screening.
基于 Rhodamine B（RB）功能化的金纳米粒子（AuNPs），开发了一种简单的方法来制备对乐果具有高选择性和高灵敏度的探针。在溶液中存在 AuNPs 的情况下，观察到 RB 荧光的猝灭，这是由荧光共振能量转移介导的。当乐果分子取代 AuNPs 表面上的 RB 分子时，乐果存在下 RB-AuNP 溶液的荧光强度逐渐恢复，这显著增加了 RB 的荧光强度。荧光与乐果浓度在 0.005-1.0 ppm 范围内呈正比（R = 0.989），LOD 为 0.004 ppm。在水和水果样品中的乐果回收率为 86-116%，具有良好的 RSD（<9.3%）。由于其高灵敏度、优异的选择性和方便的制造工艺，该方法是乐果筛选的有前途的候选方法。
