Dong Liang, Hou Changjun, Fa Huanbao, Yang Mei, Wu Huixiang, Zhang Liang, Huo Danqun
Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
J Nanosci Nanotechnol. 2018 Apr 1;18(4):2441-2449. doi: 10.1166/jnn.2018.14283.
Cartap residue poses a great threat to human health and its derivatives would remain in soils, natural waters and other environmental domains for a long time. Herein, a simple, rapid and ultrasensitive analytical method for the determination of cartap based on fluorescence resonance energy transfer (FRET) between Au nanoparticles (AuNPs) and rhodamine B (RB) is first described. With the presence of citrate-stabilized AuNPs, the fluorescence of RB was remarkably quenched by AuNPs via FRET. The fluorescence of the AuNPs-RB system was recovered upon addition of cartap, cartap can be adsorbed on the surface of AuNPs due to its amino group that has good affinity with gold, which could induce the aggregation of AuNPs accompanying color change from red to blue. Thus, the FRET between AuNPs and RB was weakened and the PL intensity of RB was recovered accordingly. A good linear correlation for detection of RB was exhibited from 1 nM to 180 nM, and the detection limit reached 0.88 nM, which was much lower than the safety limit required by USA, UK and China. To the best of our knowledge, it has been the lowest detection ever without the aid of costly instrumentation. This method was successfully carried out for the assessment of cartap in real samples with satisfactory results, which revealed many advantages such as high sensitivity, low cost and non-time-consuming compared with traditional methods.
杀螟丹残留对人类健康构成巨大威胁，其衍生物会长期残留在土壤、天然水体及其他环境领域中。在此，首次描述了一种基于金纳米颗粒（AuNPs）与罗丹明B（RB）之间的荧光共振能量转移（FRET）来测定杀螟丹的简单、快速且超灵敏的分析方法。在柠檬酸盐稳定的AuNPs存在下，RB的荧光通过FRET被AuNPs显著猝灭。加入杀螟丹后，AuNPs-RB体系的荧光恢复，杀螟丹因其与金具有良好亲和力的氨基可吸附在AuNPs表面，这会导致AuNPs聚集，颜色从红色变为蓝色。因此，AuNPs与RB之间的FRET减弱，RB的荧光强度相应恢复。检测RB时，在1 nM至180 nM范围内呈现良好的线性相关性，检测限达到0.88 nM，远低于美国、英国和中国要求的安全限值。据我们所知，这是在不借助昂贵仪器的情况下有史以来最低的检测限。该方法成功用于实际样品中杀螟丹的评估，结果令人满意，与传统方法相比，显示出许多优点，如高灵敏度、低成本和不耗时。
