Department of Interventional Radiology and Vascular Surgery, The First Affiliated Hospital of Jinan University, No. 613 of West Huangpu Avenue, Guangzhou, 510630, China.
Department of Interventional Radiology and Vascular Surgery, The First Affiliated Hospital of Jinan University, No. 613 of West Huangpu Avenue, Guangzhou, 510630, China.
Acad Radiol. 2018 Dec;25(12):1609-1616. doi: 10.1016/j.acra.2018.03.020. Epub 2018 Apr 23.
This paper describes an ongoing investigation of imaging and characterization of ischemia-reperfusion (IR) and investigated the use of color-coded digital subtraction angiography (DSA) to assess reperfusion injury or potential injury.
New Zealand white rabbits were subjected to right hindlimb ischemia (IR, n = 24) or sham operation (control, n = 6). After 3 hours, the IR rabbits underwent reperfusion and were assessed at 0, 6, 12, or 24 hours (n = 6 each). DSA of the bilateral vastus lateralis muscle of each animal was performed. The maximum contrast enhancement value of a consistent region of interest in the right and left hind limbs (peak enhancement-R/L) was determined. Associations between the relative ratio of the peak right limb to the peak left limb (peak-R/L) and the following blood indicators of IR injury were analyzed: lactic dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA), and superoxide dismutase (SOD).
Serum LDH, CK, and MDA values in each IR group were significantly higher than those of the control group and were positively associated with the IR interval, whereas SOD was significantly lower and negatively associated. The mean peak-R/L decreased linearly with the IR interval from 1.07 ± 0.01 in the control group, and 0.93 ± 0.06, 0.79 ± 0.05, 0.65 ± 0.04, and 0.47 ± 0.04 at 0, 6, 12, and 24 hours in the IR groups. The coefficients of correlation between the peak-R/L and LDH, CK, MDA, SOD serum levels were -0.885, -0.908, -0.541, and 0.832, respectively.
Color-coded DSA may be used for monitoring the dynamics of skeletal muscle IR injury.
本文描述了一项正在进行的关于成像和缺血再灌注（IR）特征的研究，并探讨了使用彩色数字减影血管造影（DSA）来评估再灌注损伤或潜在损伤的方法。
将新西兰大白兔的右后肢（IR，n=24）或假手术（对照，n=6）。在 3 小时后，IR 兔进行再灌注，并在 0、6、12 或 24 小时（每组 n=6）进行评估。对每只动物的双侧股外侧肌进行 DSA。确定右侧和左侧后肢的一致感兴趣区域的最大对比度增强值（峰值增强-R/L）。分析右侧与左侧峰值的相对比值（峰值-R/L）与以下反映 IR 损伤的血液指标之间的相关性：乳酸脱氢酶（LDH）、肌酸激酶（CK）、丙二醛（MDA）和超氧化物歧化酶（SOD）。
每组 IR 组的血清 LDH、CK 和 MDA 值均明显高于对照组，且与 IR 间隔呈正相关，而 SOD 则明显降低且与 IR 间隔呈负相关。平均峰值-R/L 与 IR 间隔呈线性下降，从对照组的 1.07±0.01，IR 组在 0、6、12 和 24 小时分别为 0.93±0.06、0.79±0.05、0.65±0.04 和 0.47±0.04。峰值-R/L 与 LDH、CK、MDA 和 SOD 血清水平的相关系数分别为-0.885、-0.908、-0.541 和 0.832。
彩色 DSA 可用于监测骨骼肌 IR 损伤的动态变化。
