Department of Interventional Radiology and Vascular Surgery, The First Affiliated Hospital of Jinan University, No.613 of West Huangpu Avenue, Guangzhou, 510630, China.
Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, China.
BMC Med Imaging. 2019 Aug 28;19(1):75. doi: 10.1186/s12880-019-0353-1.
In recent years, skeletal muscle-related ischemia-reperfusion injury (IRI) has become more common. IRI can lead to severe limb injuries, multiple organ failure, and even death in some cases. However, there is still a lack of rapid and sensitive detection methods for IRI in skeletal muscle. This study aims to explore the value of computed tomography perfusion imaging (CTPI) and color-coded digital subtraction angiography (DSA) in assessing acute IRI of skeletal muscle in a rabbit model.
Fifty New Zealand white rabbits were randomly assigned to the ischemia-reperfusion (IR) group (n = 40) or sham group (n = 10). After 3 h of surgically-induced hindlimb ischemia, the IR group underwent reperfusion and CTPI and color-coded DSA were taken to assess the skeletal muscle at 0, 6, 12, or 24 h post-reperfusion. The data from CTPI and DSA in the right and left hindlimbs, blood flow (AF-R/L), blood volume (BV-R/L), contrast clearance rate (C-R/L) and the maximum contrast enhancement values (peak-R/L) were obtained. Serum superoxide dismutase (SOD), creatine kinase (CK), lactic dehydrogenase (LDH) and malondialdehyde (MDA) were measured. The statistical correlation between the above parameters (CTPI, color-coded DSA, and biochemical markers) was analyzed.
The mean value of AF-R/L, BV-R/L, C-R/L and peak-R/L decreased linearly from 1.07 ± 0.08 to 0.75 ± 0.11, 1.03 ± 0.06 to 0.85 ± 0.14, 0.93 ± 0.15 to 0.71 ± 0.18, and 1.07 ± 0.01 to 0.47 ± 0.04, respectively. The correlation coefficients between AF-R/L and SOD, CK, LDH and MDA were 0.57, - 0.44, - 0.60, and - 0.62, respectively (p < 0.001). The correlation coefficients between Peak-R/L and SOD, CK, LDH, MDA were 0.59, 0.68, 0.71 and 0.66, respectively (p < 0.001). The correlation coefficient between AF-R/L and Peak-R/L was 0.70 (p < 0.001).
Both CTPI and color-coded DSA could dynamically assess skeletal muscle IRI in rabbits.
近年来，骨骼肌相关的缺血再灌注损伤（IRI）越来越常见。IRI 可导致严重的肢体损伤、多器官衰竭，甚至在某些情况下导致死亡。然而，骨骼肌 IRI 仍然缺乏快速和敏感的检测方法。本研究旨在探讨计算机断层灌注成像（CTPI）和彩色数字减影血管造影（DSA）在评估兔骨骼肌急性 IRI 中的价值。
50 只新西兰白兔随机分为缺血再灌注（IR）组（n=40）和假手术组（n=10）。手术诱导后 3 小时下肢缺血，IR 组再灌注，分别在再灌注后 0、6、12 或 24 小时进行 CTPI 和彩色编码 DSA 评估骨骼肌。获得右、左后肢 CTPI 和 DSA 的血流量（AF-R/L）、血容量（BV-R/L）、对比清除率（C-R/L）和最大对比增强值（peak-R/L）。检测血清超氧化物歧化酶（SOD）、肌酸激酶（CK）、乳酸脱氢酶（LDH）和丙二醛（MDA）。分析上述参数（CTPI、彩色编码 DSA 和生化标志物）之间的统计学相关性。
AF-R/L、BV-R/L、C-R/L 和 peak-R/L 的平均值从 1.07±0.08 线性下降至 0.75±0.11、1.03±0.06 至 0.85±0.14、0.93±0.15 至 0.71±0.18 和 1.07±0.01 至 0.47±0.04。AF-R/L 与 SOD、CK、LDH 和 MDA 的相关系数分别为 0.57、-0.44、-0.60 和-0.62（p<0.001）。Peak-R/L 与 SOD、CK、LDH、MDA 的相关系数分别为 0.59、0.68、0.71 和 0.66（p<0.001）。AF-R/L 与 Peak-R/L 的相关系数为 0.70（p<0.001）。
CTPI 和彩色编码 DSA 均可动态评估兔骨骼肌 IRI。
