Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, PR China; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, 60612, USA.
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, 60612, USA; UIC/NIH Center for Botanical Dietary Supplements Research, Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, 60612, USA.
J Chromatogr A. 2018 Jun 8;1553:108-115. doi: 10.1016/j.chroma.2018.04.033. Epub 2018 Apr 21.
Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds.
逆流分离（CCS）已广泛用于分离高丰度化合物。然而，从天然来源中鉴定低丰度化合物，如哺乳动物甾体激素，仍然是一项具有挑战性的任务。为开发 CCS 富集策略，制备了 14 种人甾体激素参考化合物的混合物。采用基于 TLC 的 GUESS（溶剂系统的通用估算）方法和分配实验，开发了一种用 CCS 富集这些低丰度化合物的方法。CCS 在牡荆属植物提取物中甾体激素的富集中的应用通过鉴定孕酮和雌三醇得到了证实。该方法提供了一种基于 CCS 的策略，用于从植物资源中挖掘低丰度化合物。
