Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Science. 2018 Jun 1;360(6392). doi: 10.1126/science.aar5780. Epub 2018 Apr 26.
Time series of single-cell transcriptome measurements can reveal dynamic features of cell differentiation pathways. From measurements of whole frog embryos spanning zygotic genome activation through early organogenesis, we derived a detailed catalog of cell states in vertebrate development and a map of differentiation across all lineages over time. The inferred map recapitulates most if not all developmental relationships and associates new regulators and marker genes with each cell state. We find that many embryonic cell states appear earlier than previously appreciated. We also assess conflicting models of neural crest development. Incorporating a matched time series of zebrafish development from a companion paper, we reveal conserved and divergent features of vertebrate early developmental gene expression programs.
单细胞转录组测量的时间序列可以揭示细胞分化途径的动态特征。通过对跨越合子基因组激活到早期器官发生的整个青蛙胚胎的测量，我们得到了脊椎动物发育中细胞状态的详细目录，以及随时间推移的所有谱系分化的图谱。推断的图谱再现了大多数（如果不是全部）发育关系，并将新的调节剂和标记基因与每个细胞状态相关联。我们发现许多胚胎细胞状态比以前认为的更早出现。我们还评估了神经嵴发育的冲突模型。结合来自一篇配套论文的斑马鱼发育的匹配时间序列，我们揭示了脊椎动物早期发育基因表达程序的保守和分歧特征。
