Department of Anatomy, Aichi Medical University, Aichi, Japan.
Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
Sci Rep. 2018 Apr 26;8(1):6627. doi: 10.1038/s41598-018-25070-y.
Cold ischemia times ranging from <6 h to as long as 24 h are generally quoted as the limits for attempting the replantation of amputated extremities. In this study, we aimed to assess the effect of hyperbaric carbon monoxide (CO) and oxygen (O) on rat limb preservation. Donor rat limbs were preserved in a chamber filled with hyperbaric CO and O for 3 days (CO + O 3 days) or 7 days (CO + O 7 days). Positive and negative control groups were created by using non-preserved limbs (NP) and limbs wrapped in saline-moistened gauze for 3 days (SMG 3 days), respectively. The survival rate of transplanted limbs at postoperative day 90 was 88% in the NP and 86% in the CO + O 3 days. The corresponding survival rate was 50% in the CO + O 7 days at postoperative day 90 but was 0% in the SMG 3 days at postoperative day 3. Muscle mass decreased in the CO + O 3 days and CO + O 7 days compared with the NP, but sciatic-tibial nerve conduction velocities did not differ. These results indicate that amputated extremities preservation with hyperbaric CO and O could extend the time limits of preservation, maintaining their viability for replantation.
冷缺血时间从 <6 小时到长达 24 小时通常被认为是尝试再植离断肢体的极限。在这项研究中，我们旨在评估高压一氧化碳 (CO) 和氧气 (O) 对大鼠肢体保存的影响。供体大鼠肢体在充满高压 CO 和 O 的腔室内保存 3 天 (CO+O 3 天) 或 7 天 (CO+O 7 天)。阳性和阴性对照组分别通过使用未保存的肢体 (NP) 和用生理盐水湿润的纱布包裹 3 天 (SMG 3 天) 来创建。术后第 90 天，NP 组和 CO+O 3 天组的移植肢体存活率分别为 88%和 86%。在 CO+O 7 天组，术后第 90 天的相应存活率为 50%，而在 SMG 3 天组，术后第 3 天的存活率为 0%。与 NP 相比，CO+O 3 天和 CO+O 7 天的肌肉质量减少，但坐骨-胫神经传导速度没有差异。这些结果表明，高压 CO 和 O 保存离断肢体可以延长保存时间，保持其再植的活力。
