Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Republic of Korea.
College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.
J Antibiot (Tokyo). 2018 Aug;71(8):753-756. doi: 10.1038/s41429-018-0058-7. Epub 2018 Apr 26.
Four new compounds, phomalichenones A-D (1-4), and seven known compounds (5-11) were isolated from the cultures of an endolichenic fungus Phoma sp. EL002650. Their structures were determined by the analysis of their spectroscopic data (NMR and MS). Compounds 1 and 6 inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In addition, compound 1 diminished the protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and decreased the mRNA expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin(IL)-1β, and IL-6.
从内生真菌 Phoma sp. EL002650 的培养物中分离得到了 4 个新化合物 phomalichenones A-D（1-4）和 7 个已知化合物（5-11）。通过光谱数据分析（NMR 和 MS）确定了它们的结构。化合物 1 和 6 抑制脂多糖（LPS）刺激的 RAW264.7 巨噬细胞中一氧化氮（NO）的产生。此外，化合物 1 降低了诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白表达水平，并降低了促炎细胞因子 TNF-α、IL-1β 和 IL-6 的 mRNA 表达水平。
