Laboratory of Food Technology, School of Chemistry and Food, Federal University of Rio Grande (FURG), Avenida Itália, km 08, Rio Grande, Rio Grande do Sul, 96201-900, Brazil.
Laboratory of Biotechnology Process and Purification of Macromolecules, Federal University of São João Del-Rey, Campus Centro Oeste, Av. Sebastião Gonçalves Coelho, 400, Divinópolis, 35501-296, Brazil.
Bioprocess Biosyst Eng. 2018 Aug;41(8):1177-1183. doi: 10.1007/s00449-018-1946-8. Epub 2018 Apr 26.
Conventional biosurfactants have high production costs. Therefore, the use of low-cost carbon sources for their production is attractive for industry. The ability to remain stable under various environmental conditions further extends industrial application. Here we aimed to evaluate the stability of a new lipopeptide produced by Corynebacterium aquaticum using fish residue as an unconventional energy source. The biosurfactant was produced using 3% fish residue, 2% of the microorganism, and mineral medium. Biosurfactant characterization was performed by thin layer chromatography (TLC), as well as by testing its infrared, surface tension, emulsifying activity, and ionic character. The stability of the biosurfactant was evaluated by testing its surface tension at a range of temperatures, pH, and saline concentrations, as well as after 6 months of storage. The biosurfactant was characterized as a lipopeptide due to its retention time, which was coincident with the amino acid and lipid chains obtained in the TLC analysis, being confirmed by some regions of absorption verified in the infrared analysis. The surface tension and emulsifying activity of the biosurfactant were 27.8 mN/m and 87.6%, respectively, and showed anionic character. The biosurfactant was stable at temperatures of 20 to 121 °C, in saline concentrations of 1 to 7%, and at pH close to neutrality. Based on our findings, it is possible to use unconventional sources of energy to produce a lipopeptide biosurfactant that can act under various environments.
传统生物表面活性剂的生产成本很高。因此，使用低成本的碳源来生产它们对工业具有吸引力。在各种环境条件下保持稳定的能力进一步扩展了其工业应用。在这里，我们旨在评估使用鱼渣作为非常规能源生产的新脂肽的稳定性。该生物表面活性剂使用 3%的鱼渣、2%的微生物和矿物质培养基进行生产。通过薄层色谱（TLC）以及测试其红外、表面张力、乳化活性和离子特性来进行生物表面活性剂的特性描述。通过测试其在一系列温度、pH 值和盐浓度下的表面张力以及在 6 个月的储存后，来评估生物表面活性剂的稳定性。该生物表面活性剂被表征为脂肽，因为其保留时间与 TLC 分析中获得的氨基酸和脂质链一致，并且在红外分析中验证了一些吸收区域得到了证实。生物表面活性剂的表面张力和乳化活性分别为 27.8 mN/m 和 87.6%，并具有阴离子特性。生物表面活性剂在 20 至 121°C 的温度下、1 至 7%的盐浓度下以及接近中性 pH 值的条件下稳定。根据我们的发现，可以使用非常规的能源来生产可以在各种环境下发挥作用的脂肽生物表面活性剂。
