Laboratory of Biomedical Technologies of Institute for Cellular and Intracellular Symbiosis UrB RAS, Orenburg, Russia.
Arch Microbiol. 2023 Apr 1;205(4):158. doi: 10.1007/s00203-023-03498-9.
Biofilm formation is an important factor in the development of antibiotic resistance and chronic infection. In this study, we demonstrated that the cell-free supernatant of vaginal isolates of C. amycolatum caused a reduction in biofilm formation, destroyed the preformed biofilms, altered the cell surface properties and reduced the production of exopolysaccharides in clinical isolates of P. aeruginosa и Kl. pneumoniae. Microscopic observations showed that P. aeruginosa and Kl. pneumoniae biofilm formed small clusters scattered over the surface after treatment with cell-free supernatant of C. amycolatum ICIS 99, in contrast to the dense aggregates observed in controls, as well as the flat, scattered, and unstructured biofilm architecture after treatment of preformed biofilms cell-free supernatant. The cells were flat and relatively unstructured. Based on these results, we hypothesize that C. amycolatum likely produces secondary metabolites with antimicrobial activity and utilizes a similar mechanism of action to bacteriocins and/or biosurfactants. The data obtained open the prospect of studying the metabolic profile of the cell-free supernatant of C. amycolatum to understand the nature and mechanism of the detected antibacterial action and provide further support for the probiotic potential of C. amycolatum vaginal isolates.
生物膜形成是抗生素耐药性和慢性感染发展的一个重要因素。在这项研究中，我们证明了无细胞的阴道分枝杆菌分离株的上清液导致生物膜形成减少，破坏了预先形成的生物膜，改变了细胞表面特性，并减少了临床分离的铜绿假单胞菌和肺炎克雷伯菌的胞外多糖的产生。显微镜观察显示，在经过无细胞的分枝杆菌 ICIS 99 上清液处理后，铜绿假单胞菌和肺炎克雷伯菌生物膜形成小的簇状，散布在表面上，与对照中观察到的密集聚集物形成对比，以及预先形成的生物膜经无细胞上清液处理后的扁平、分散和无结构的生物膜结构。细胞是平的，相对没有结构。基于这些结果，我们假设分枝杆菌可能产生具有抗菌活性的次级代谢产物，并利用类似于细菌素和/或生物表面活性剂的作用机制。获得的数据为研究无细胞的分枝杆菌上清液的代谢谱开辟了前景，以了解检测到的抗菌作用的性质和机制，并为阴道分枝杆菌分离株的益生菌潜力提供了进一步的支持。
