Tremoen Nina Hårdnes, Gaustad Ann Helen, Andersen-Ranberg Ina, van Son Maren, Zeremichael Teklu Tewoldebrhan, Frydenlund Kirsten, Grindflek Eli, Våge Dag Inge, Myromslien Frøydis Deinboll
Department of Biotechnology, Inland Norway University of Applied Sciences, NO-2318, Hamar, Norway; Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432, Ås, Norway.
Department of Biotechnology, Inland Norway University of Applied Sciences, NO-2318, Hamar, Norway; Topigs Norsvin, Storhamargata 44, NO-2317, Hamar, Norway.
Anim Reprod Sci. 2018 Jun;193:226-234. doi: 10.1016/j.anireprosci.2018.04.075. Epub 2018 Apr 19.
Boar fertility has a major impact on overall pig reproductive efficiency. Using accurate and objective in vitro sperm variables for predicting in vivo fertility from a single ejaculate, however, is challenging. Motility is the most widely used indicator of sperm quality, and a computer assisted sperm analysis (CASA) system is now available for objective assessment of sperm motility characteristics. In this study sperm motility characteristics and semen ATP concentrations were investigated and the effect of both were evaluated on total number of piglets born (TNB) when Norwegian Landrace (NL) and Norwegian Duroc (ND) boar semen was used for AI. In addition, breed differences for semen storage capacity were investigated. The results from CASA analysis indicated there were differences between NL and ND sperm motility variables. The percentage of motile sperm cells decreased in both NL (P = 0.01) and ND (P < 0.0001) during storage. A large proportion of sperm cells with a hyperactive motility pattern were detected in ND semen on the day of collection, with no significant changes as a result of storage. Inconsistent with this finding, there was greater degree of hyper-activation in sperm motility pattern for NL because of semen storage. There was a significant decrease in semen ATP concentration during storage (P < 0.0001) in both breeds. The linearity of sperm movement at the day of collection and the wobble after storage influenced TNB in NL, while the percentage of motile cells, curvilinear velocity and lateral head amplitude on the day of semen collection and linearity after storage influenced TNB in ND.
公猪的繁殖力对猪的整体繁殖效率有重大影响。然而，利用准确、客观的体外精子变量来预测单次射精的体内繁殖力具有挑战性。活力是最广泛使用的精子质量指标，目前有一种计算机辅助精子分析（CASA）系统可用于客观评估精子活力特征。在本研究中，对挪威长白猪（NL）和挪威杜洛克猪（ND）精液用于人工授精时的精子活力特征和精液ATP浓度进行了研究，并评估了两者对产仔总数（TNB）的影响。此外，还研究了精液储存能力的品种差异。CASA分析结果表明，NL和ND的精子活力变量存在差异。在储存期间，NL（P = 0.01）和ND（P < 0.0001）的活动精子细胞百分比均下降。在采集当天，ND精液中检测到很大比例的具有活跃运动模式的精子细胞，储存后无显著变化。与这一发现不一致的是，由于精液储存，NL精子运动模式的超激活程度更大。两个品种在储存期间精液ATP浓度均显著下降（P < 0.0001）。采集当天精子运动的线性度和储存后的摆动影响NL的TNB，而精液采集当天的活动细胞百分比、曲线速度和头部侧向幅度以及储存后的线性度影响ND的TNB。
