Myromslien Frøydis D, Tremoen Nina H, Andersen-Ranberg Ina, Fransplass Ragnhild, Stenseth Else-Berit, Zeremichael Teklu T, van Son Maren, Grindflek Eli, Gaustad Ann Helen
Department of Biotechnology, Inland Norway University of Applied Sciences, Hamar, Norway.
Topigs Norsvin, Hamar, Norway.
Reprod Domest Anim. 2019 Feb;54(2):160-166. doi: 10.1111/rda.13322. Epub 2018 Sep 29.
The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n = 693) and Norwegian Duroc (ND; n = 655), and to evaluate the influence on total number of piglets born (TNB). There was a significantly higher median DFI (p < 0.0001) in ejaculates from the 478 ND boars compared to the 452 NL boars. Data from 19,496 NL litters and 3,877 ND litters of the same boars were retrieved. For either breed, sow herd (p < 0.0001), parity (p < 0.05) and DFI (p < 0.05) showed significant effects on TNB. The DFI was negatively correlated to TNB in both breeds. The boars with the 5% lowest TNB had a least square means DFI of 3.05% and 2.24% in NL and ND, respectively, compared to 1.67% and 1.23% for the boars with the 5% highest TNB (p < 0.01). The DFI and the motility of the same semen samples were negatively correlated (p < 0.0001), and the high and low TNB groups showed significant differences in motility. However, this difference could not be used for practical prediction of TNB group (92.1% vs. 89.7%; p = 0.0038 and 92.3% vs. 89.5%; p = 0.018; NL and ND, respectively). In conclusion, our results indicate that sperm DNA integrity in semen with good motility and morphology may be an additional prediction parameter for fertility in pigs.
精子染色质结构分析是一种评估精子DNA碎片化的方法，据报道，该参数在几种哺乳动物中与田间生育能力呈负相关。该方法计算出一个DNA碎片化指数（DFI），其高值表明染色质结构异常。在这项从2010年3月持续到2017年6月的研究中，目的是评估两种不同猪品种（挪威长白猪（NL；n = 693）和挪威杜洛克猪（ND；n = 655））的储存液态稀释精液中的精子DFI，并评估其对产仔总数（TNB）的影响。与452头NL公猪相比，478头ND公猪的射精中DFI中位数显著更高（p < 0.0001）。检索了同一公猪的19496窝NL仔猪和3877窝ND仔猪的数据。对于任一品种，母猪群（p < 0.0001）、胎次（p < 0.05）和DFI（p < 0.05）对TNB均有显著影响。两个品种中DFI均与TNB呈负相关。TNB最低的5%的公猪，NL和ND的最小二乘均值DFI分别为3.05%和2.24%，而TNB最高的5%的公猪分别为1.67%和1.23%（p < 0.01）。同一精液样本的DFI与活力呈负相关（p < 0.0001），TNB高低组在活力上有显著差异。然而，这种差异不能用于实际预测TNB组（分别为92.1%对89.7%；p = 0.0038和92.3%对89.5%；p = 0.018；NL和ND）。总之，我们的结果表明，具有良好活力和形态的精液中的精子DNA完整性可能是猪生育能力的一个额外预测参数。
