Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan.
J Proteomics. 2018 Jun 15;181:238-248. doi: 10.1016/j.jprot.2018.04.031. Epub 2018 Apr 25.
Flooding negatively affects the growth of soybean, whereas the plant-derived smoke enhances seedling growth of crops. To clarify the mechanism underlying the recovery from flooding stress, proteomic analysis was performed based on morphological results. Growth of soybean seedlings was inhibited under flooding stress, but it recovered after water removal following treatment with plant-derived smoke. Sucrose/starch metabolism and glycolysis were suppressed in smoke-treated flooded soybean compared to flooded soybean. The protein abundance and gene expression of O-fucosyltransferase family proteins related to the cell wall were higher in smoke-treated flooded soybean than in flooded soybean. Protein abundance and gene expression of peptidyl-prolyl cis-trans isomerase and Bowman-Birk proteinase isoinhibitor D-II were lower in smoke-treated flooded soybean than in flooded soybean. Taken together, these results suggest that plant-derived smoke enhances soybean growth during recovery from flooding stress through the balance of sucrose/starch metabolism and glycolysis. Furthermore, the accumulation of cell-wall related protein might be an important factor contributing to recovery of soybean from flooding stress.
Flooding negatively affects the growth of soybean, whereas the plant-derived smoke enhances the seedling growth of crops. To clarify the mechanism underlying the recovery from flooding stress, proteomic analysis of soybean with different treatments including normal conditions, flooding stress, and flooding stress in the presence of plant-derived smoke was performed in this study. Growth of soybean seedlings was inhibited under flooding stress, however, it recovered with plant-derived smoke treatment during recovery from flooding stress. Sucrose/starch metabolism and glycolysis were suppressed in smoke-treated flooded soybean compared to flooded soybean, which suggests altered sucrose/starch metabolism and glycolysis contribute to soybean growth recovery from flood stress. Furthermore, the protein abundance and gene expression of O-fucosyltransferase family proteins related to the cell wall was higher in smoke-treated flooded soybean than in flooded soybean, which might be an important factor contributing to the recovery of soybean from flooding stress.
洪水会对大豆的生长产生负面影响，而植物衍生的烟雾则会促进作物幼苗的生长。为了阐明从洪水胁迫中恢复的机制，根据形态学结果进行了蛋白质组学分析。大豆幼苗在洪水胁迫下生长受到抑制，但在烟雾处理后去除水分后恢复生长。与受洪水影响的大豆相比，烟雾处理的受洪水影响的大豆中的蔗糖/淀粉代谢和糖酵解受到抑制。与受洪水影响的大豆相比，烟雾处理的受洪水影响的大豆中与细胞壁相关的 O-岩藻糖基转移酶家族蛋白的蛋白质丰度和基因表达更高。烟雾处理的受洪水影响的大豆中的肽基脯氨酰顺反异构酶和 Bowman-Birk 蛋白酶同工抑制剂 D-II 的蛋白质丰度和基因表达低于受洪水影响的大豆。总的来说，这些结果表明，植物衍生的烟雾通过蔗糖/淀粉代谢和糖酵解的平衡增强了大豆在从洪水胁迫中恢复时的生长。此外，细胞壁相关蛋白的积累可能是大豆从洪水胁迫中恢复的一个重要因素。
洪水会对大豆的生长产生负面影响，而植物衍生的烟雾则会促进作物幼苗的生长。为了阐明从洪水胁迫中恢复的机制，本研究对包括正常条件、洪水胁迫和存在植物衍生烟雾的洪水胁迫下的大豆进行了不同处理的蛋白质组学分析。大豆幼苗在洪水胁迫下生长受到抑制，但在烟雾处理后从洪水胁迫中恢复时，生长得到恢复。与受洪水影响的大豆相比，烟雾处理的受洪水影响的大豆中的蔗糖/淀粉代谢和糖酵解受到抑制，这表明蔗糖/淀粉代谢和糖酵解的改变有助于大豆从洪水胁迫中恢复生长。此外，与受洪水影响的大豆相比，烟雾处理的受洪水影响的大豆中与细胞壁相关的 O-岩藻糖基转移酶家族蛋白的蛋白质丰度和基因表达更高，这可能是大豆从洪水胁迫中恢复的一个重要因素。
