Young Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran.
Department of Chemistry, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran.
Ultrason Sonochem. 2018 Jul;45:106-115. doi: 10.1016/j.ultsonch.2018.03.009. Epub 2018 Mar 17.
In this study, silver nanoparticles modified choline chloride functionalized graphene oxide (AgNPs-ChCl-GO) was synthesized using sonochemical method and utilized as a bioelectrochemical sensor for detection of celecoxib (CEL). The characterization studies were ultimately performed in order to acheive a more complete understanding of the morphological and structural features of the AgNPs-ChCl-GO using different techniques including FT-IR, AFM, FE-SEM, EDX, and XRD. AgNPs-ChCl-GO demonstrated a significant improvement in the reduction activity of CEL due to the enhancement in the current response compared to the bare carbon paste electrode (CPE). The optimum experimental conditions, were optimized using central composite design (CCD) methodology. The differential pulse voltammetry (DPVs) showed an expanded linear dynamic ranges of 9.6 × 10-7.4 × 10 M for celecoxib in Britton-Robinson buffer in pH 5.0 with. LOD (S/N = 3) and LOQ (S/N = 10) were obtained 2.51 × 10 M and 6.58 × 10 M respectively. AgNPs-ChCl-GO-carbon paste electrode exhibited suitable properties and high accuracy determination of celecoxib in the human plasma sample.
在这项研究中，采用超声化学法合成了银纳米粒子修饰的氯化胆碱功能化氧化石墨烯（AgNPs-ChCl-GO），并将其用作检测塞来昔布（CEL）的生物电化学传感器。最终进行了表征研究，以便使用不同的技术（包括 FT-IR、AFM、FE-SEM、EDX 和 XRD）更全面地了解 AgNPs-ChCl-GO 的形态和结构特征。与裸碳糊电极（CPE）相比，AgNPs-ChCl-GO 由于电流响应增强，对 CEL 的还原活性有了显著提高。使用中心复合设计（CCD）方法优化了最佳实验条件。差分脉冲伏安法（DPVs）显示，在 pH 5.0 的 Britton-Robinson 缓冲液中，塞来昔布的线性动态范围扩展到 9.6×10-7.4×10 M，检测限（S/N=3）和定量限（S/N=10）分别为 2.51×10 M 和 6.58×10 M。AgNPs-ChCl-GO-碳糊电极在人血浆样品中对塞来昔布具有合适的性质和高精度的测定。
