You Xiaojuan, Shao Mingyi, Wang Huadong, Zhu Rui, Liu Xinwei, Dong Lei, Gong Yuesheng, Li Yongwei
Central Laboratory, Zhengzhou Anorectal Hospital, Zhengzhou, China.
The First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, China.
Front Bioeng Biotechnol. 2025 Jul 9;13:1619336. doi: 10.3389/fbioe.2025.1619336. eCollection 2025.
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect in a wide concentration range (10 CFU mL-10 CFU mL) with a low detection limit of 3 CFU mL. What's more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.
幽门螺杆菌是胃炎和胃溃疡的主要病因之一。早期检测对于预防胃癌具有重要意义。在此，建立了一种以AgNPs-GO作为氧化还原探针的灵敏电化学适体传感器，用于血清和粪便样本中幽门螺杆菌的特异性检测。首先，以葡萄糖为还原剂，通过在氧化石墨烯（GO）表面还原AgNPs获得AgNPs-GO纳米复合材料，其中AgNPs具有良好的生物相容性和化学稳定性以及氧化还原特性，而GO提供了大表面积以组装大量AgNPs。随后，金纳米粒子的电沉积进一步提高了适体传感器的导电性。此外，将链霉亲和素引入适体传感器以有效结合生物素修饰的适体。通过这种方式，适体可以通过SA-生物素连接固定在表面，随后幽门螺杆菌被适体选择性结合。在最佳条件下，该适体传感器能够在宽浓度范围（10 CFU mL-10 CFU mL）内检测幽门螺杆菌，检测限低至3 CFU mL。此外，所开发的方法在实际应用中表现出优异的性能，为临床诊断中其他病原体的检测提供了有前景的可能性。
