Shimizu M, Nakamura M, Kataoka T, Iwaguchi T
Department of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan.
J Pharmacobiodyn. 1988 Mar;11(3):152-7. doi: 10.1248/bpb1978.11.152.
The splenic T-cells of concanavalin A (Con-A)-injected mice suppressed the in vitro Con A-induced splenic blastogenesis of normal mice. We examined electrophoretic mobilities (EPM) and surface markers of Con A-induced suppressor T-cells as a model of suppressor T-cells. The EPM of the suppressor cells with both Thy-1 and gangliotetraosyl-ceramide (asialo GM1) was lower than that of normal T-cells. The ratio of low mobility T-cells (LMT) to high mobility T-cells (HMT) correlated significantly with dose-response and time-course of Con A in the suppressor activity and with the percentages of cells carrying asialo GM1 antigen and receptor for peanut agglutinin. For in vitro induction by Con A, Lyt-2+ cells were reported to have suppressor activity. However, for in vivo induction by Con A, Lyt-2+ cells did not correlate with the suppressor activity in the dose-response and time-course experiments. By separating the Lyt-subsets, it was found that both Lyt-1+2+ and Lyt-1+2- subsets were suppressor cells in the blastogenesis. The suppressor activity of in vivo Con A-induced spleen cells correlated with the LMT/HMT ratio, but not with the analysis of Lyt-phenotype. These results suggested that lymphocytes electrophoresis clearly distinguished Con A-induced suppressor T-cells with low EPM from normal T-cells.
注射伴刀豆球蛋白A（Con-A）的小鼠的脾T细胞抑制了正常小鼠体外Con A诱导的脾细胞增殖。我们检测了Con A诱导的抑制性T细胞作为抑制性T细胞模型的电泳迁移率（EPM）和表面标志物。同时表达Thy-1和神经节四糖神经酰胺（脱唾液酸GM1）的抑制性细胞的EPM低于正常T细胞。低迁移率T细胞（LMT）与高迁移率T细胞（HMT）的比例与Con A在抑制活性方面的剂量反应和时间进程以及携带脱唾液酸GM1抗原和花生凝集素受体的细胞百分比显著相关。据报道，对于Con A的体外诱导，Lyt-2+细胞具有抑制活性。然而，对于Con A的体内诱导，在剂量反应和时间进程实验中，Lyt-2+细胞与抑制活性无关。通过分离Lyt亚群，发现在细胞增殖中Lyt-1+2+和Lyt-1+2-亚群都是抑制性细胞。体内Con A诱导的脾细胞的抑制活性与LMT/HMT比例相关，但与Lyt表型分析无关。这些结果表明，淋巴细胞电泳能清楚地将Con A诱导的低EPM抑制性T细胞与正常T细胞区分开来。
