Shimizu M, Sekine K, Kataoka T, Iwaguchi T
Department of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan.
Int J Immunopharmacol. 1990;12(5):545-51. doi: 10.1016/0192-0561(90)90119-8.
We have previously reported that concanavalin A (Con A)-induced suppressor T-cells in vivo are located in the lower electrophoretic mobility (EPM) zone as judged by the ratio of low mobility T-cells to high mobility T-cells (LMT/HMT ratio) in terms of lymphocyte electrophoresis, and that the administration of 5-fluorouracil (5-FU) eliminates their suppressor activity as assayed by Con A-induced lymphocyte blastogenesis in vitro. The current study was undertaken to clarify the relation between changes in EPM and suppressor activity of Con A-induced suppressor T-cells by the administration of antitumor drugs such as cyclophosphamide (CY) and 5-FU when assayed by production of anti-sheep red blood cell antibody in vitro. The pretreatment with 5-FU, but not CY, abrogated the suppressor activity of Con A-induced T-cells followed by restoration of the LMT/HMT ratio of Con A-induced T-cells to that of normal T-cells. These finding suggest that lymphocyte electrophoresis can be applied to an assay for screening immunomodulating agents in Con A-induced suppressor T-cells.
我们之前报道过，通过淋巴细胞电泳中低迁移率T细胞与高迁移率T细胞的比例（LMT/HMT比例）判断，体内伴刀豆球蛋白A（Con A）诱导的抑制性T细胞位于较低的电泳迁移率（EPM）区，并且5-氟尿嘧啶（5-FU）的给药消除了它们的抑制活性，这是通过体外Con A诱导的淋巴细胞增殖试验测定的。当前研究旨在通过体外抗绵羊红细胞抗体产生试验，阐明给予环磷酰胺（CY）和5-FU等抗肿瘤药物时，EPM变化与Con A诱导的抑制性T细胞抑制活性之间的关系。5-FU预处理而非CY预处理消除了Con A诱导的T细胞的抑制活性，随后Con A诱导的T细胞的LMT/HMT比例恢复到正常T细胞的水平。这些发现表明，淋巴细胞电泳可用于筛选Con A诱导的抑制性T细胞中的免疫调节药物。
