Laboratorio de Análisis Clínico, Facultad de Ciencias Químicas, Saltillo, Universidad Autónoma de Coahuila, Coahuila, México.
Departamento de Ciencias Básicas, División de Ciencias de la Salud, Universidad de Monterrey, San Pedro Garza García, Nuevo León, México.
Arch Med Res. 2018 Feb;49(2):94-100. doi: 10.1016/j.arcmed.2018.04.013. Epub 2018 Apr 26.
Obesity is a complex, chronic, and multifactorial disease that has become a major, and worldwide, public health problem contributing to an increased number of pathologies, including type 2 diabetes, cardiovascular disease, hyperlipidemia, and metabolic syndrome, thus suggesting a commolon origin. A diet high in sugar and fats coupled with a sedentary lifestyle has a major role in the development of obesity. However, the genetic background has also been associated with body fat accumulation. The aim of this study was to assess the effect ofACE-rs4646994, APOA5-rs662799, and MTP-rs1800591 gene polymorphisms on clinical and biochemical parameters and to evaluate the association with body phenotypes in children and adolescent population of Saltillo, Coahuila, Mexico.
Anthropometric, clinical, biochemical parameters and BMI were obtained from 405 children and adolescents. The BMI was used to determine the body phenotype. The rs4646994 gene polymorphism was determined by PCR, whereas rs662799 and rs1800591 were determined by PCR-RFLP. The obtained results were analyzed to determine their association of these single nucleotide polymorphisms with body phenotype and biochemical parameters.
TT genotype for APOA5-rs662799 was associated with increased levels of HDL-C in the analyzed population (p <0.05). The ACErs4646994gene polymorphism is associated with high Insulin levels, HOMAIR index, and triglyceride levels, mainly when presenting a I/I genotype (p <0.05).
The polymorphic allele of the ACE gene is capable of modulating triglyceride levels, insulin levels and HOMA-IR index in the evaluated population; it must be highlighted that this has not been reported in other studied populations elsewhere.
肥胖是一种复杂的、慢性的、多因素的疾病，已成为一个主要的、全球性的公共卫生问题，导致了包括 2 型糖尿病、心血管疾病、高脂血症和代谢综合征在内的多种疾病的发病率增加，这表明其具有共同的起源。高糖和高脂肪的饮食加上久坐的生活方式在肥胖的发展中起着重要作用。然而，遗传背景也与体脂积累有关。本研究旨在评估 ACE-rs4646994、APOA5-rs662799 和 MTP-rs1800591 基因多态性对临床和生化参数的影响，并评估其与墨西哥科阿韦拉州萨尔蒂略儿童和青少年人群的体型表型的相关性。
从 405 名儿童和青少年中获得了人体测量学、临床、生化参数和 BMI。使用 BMI 来确定体型表型。通过 PCR 确定 rs4646994 基因多态性，而 rs662799 和 rs1800591 则通过 PCR-RFLP 确定。分析获得的结果，以确定这些单核苷酸多态性与体型表型和生化参数的相关性。
APOA5-rs662799 的 TT 基因型与分析人群中 HDL-C 水平升高有关（p <0.05）。ACE-rs4646994 基因多态性与高胰岛素水平、HOMAIR 指数和甘油三酯水平相关，主要在存在 I/I 基因型时（p <0.05）。
ACE 基因的多态性等位基因能够调节评估人群中的甘油三酯水平、胰岛素水平和 HOMA-IR 指数；值得强调的是，这在其他研究人群中尚未报道过。
