虾青素对奥硝唑诱导的少弱精子症大鼠附睾氧化损伤的保护作用
[Protective effect of astaxanthin against epididymal oxidative damagein rats with ornidazole-induced oligoasthenozoospermia].
作者信息
Liu Wei, Kang Xiao-Fang, Zhang Guo-Wei, Cai Hong-Cai, Li Kai-Qiang, Wang Ling-Ling, Shang Xue-Jun
机构信息
Department of Andrology, Nanjing School of Clinical Medicine, Southern Medical University/ Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Department of Hematology,The First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
出版信息
Zhonghua Nan Ke Xue. 2017 Mar;23(3):206-211.
OBJECTIVE
To investigate the improving effect of astaxanthin (AST) on the sperm quality of rats with ornidazole (ORN)-induced oligoasthenozoospermiaand its action mechanism.
METHODS
Forty adult male SD rats were equally randomized into groups A (solvent control), B (low-dose ORN ［400 mg/（kg·d）］), C (high-dose ORN ［800 mg/（kg·d）］), D (low-dose ORN ［400 mg/（kg·d）］ + AST ［20 mg/（kg·d）］), and E (high-dose ORN ［800 mg/（kg·d）］ + AST ［20 mg/（kg·d）］), all treated intragastrically for3 weeks.After treatment, the epididymal tails ononeside was taken for determination of sperm concentration and activity, and the epididymideson the other side harvested for measurement of the activities of GSH-Px, GR, CAT and SOD and the MDA contentin the homogenate.
RESULTS
Compared with group A, sperm motilityin the epididymal tail andGSH-Px and SOD activities in theepididymiswere markedly decreased while the MDAcontent significantlyincreased in group B (P<0.05), spermmotility and concentrationin the epididymal tail, testisindex, and the activities of GSH-Px, GR, CAT and SOD in the epididymis were remarkably reduced while theMDA contentsignificantly increased in group C(P<0.05). In comparison with group B, group D showed markedly increased sperm motility (［45.3±8.7］% vs ［66.3±8.9］%, P<0.05) in the epididymal tail and SOD activity in the epididymis (［116.7±25.3］ U/mg prot vs ［146.1±23.8］ U/mg prot, P<0.05), decreased MDA content(［1.68±0.45］ nmol/mg prot vs ［1.19±0.42］ nmol/mg prot, P<0.05).Compared with group C, group Eexhibited significant increases in the weight gained (［89.0±9.5］ vs ［99.9±4.1］ %, P<0.05) and sperm motility (［17.9±3.5］% vs ［27.3±5.3］ %, P<0.05) but a decrease in the content of MDA (［2.03±0.30］ nmol/mg prot vs ［1.52±0.41］ nmol/mg prot, P<0.05).
CONCLUSIONS
AST can improve spermquality in rats with ORN-inducedoligoasthenozoospermia, which may be associated with its enhancing effect on the antioxidant capacity of the epididymis.
目的
探讨虾青素（AST）对奥硝唑（ORN）诱导的少弱精子症大鼠精子质量的改善作用及其作用机制。
方法
将40只成年雄性SD大鼠随机均分为A组（溶剂对照组）、B组（低剂量ORN［400 mg/（kg·d）］）、C组（高剂量ORN［800 mg/（kg·d）］）、D组（低剂量ORN［400 mg/（kg·d）］＋AST［20 mg/（kg·d）］）和E组（高剂量ORN［800 mg/（kg·d）］＋AST［20 mg/（kg·d）］），均灌胃给药3周。治疗后，取一侧附睾尾测定精子浓度和活力，取另一侧附睾测定匀浆中谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GR）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。
结果
与A组比较，B组附睾尾精子活力、附睾中GSH-Px和SOD活性显著降低，MDA含量显著升高（P<0.05）；C组附睾尾精子活力和浓度、睾丸指数及附睾中GSH-Px、GR、CAT和SOD活性显著降低，MDA含量显著升高（P<0.05）。与B组比较，D组附睾尾精子活力明显升高（［45.3±8.7］% 比 ［66.3±8.9］%，P<0.05），附睾中SOD活性升高（［116.7±25.3］ U/mg prot比［146.1±23.8］ U/mg prot, P<0.05），MDA含量降低（［1.68±0.45］ nmol/mg prot比［1.19±0.42］ nmol/mg prot, P<0.05）。与C组比较，E组体重增加幅度显著增大（［89.0±9.5］%比［99.9±4.1］%，P<0.05），精子活力升高（［17.9±3.5］%比［27.3±5.3］%，P<0.05），MDA含量降低（［2.03±0.30］ nmol/mg prot比［1.52±0.41］ nmol/mg prot, P<0.05）。
结论
AST可改善ORN诱导的少弱精子症大鼠的精子质量，这可能与其增强附睾抗氧化能力有关。
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