Guo Bo-da, Zeng Yin, Guo Jun, Han Qiang, Wang Fu, Yu Guo-Jin, Yan Bin, Liu Sheng-Jing, Gao Qing-He
Graduate School, Peking Union Medical College, Beijing 100730, China.
Department of Urology, Beijing Hospital / National Center of Gerontology, Beijing 100730, China.
Zhonghua Nan Ke Xue. 2020 May;26(5):446-451.
To explore the possible mechanism of Huanshao Capsules (HSC) protecting the reproductive function in rats with ornidazole-induced asthenozoospermia (AZS).
Forty SD male rats were randomly divided into four groups of equal number, blank control, AZS model control, HSC and L-carnitine (LC) intervention. The AZS model was established in the latter three groups of rats by intragastrical administration of ornidazole at 400 mg/kg/d for 28 days, and meanwhile the animals in the HSC and LC groups were treated by gavage of HSC at 0.31 g/kg/d and LC at 100 mg/kg/d, respectively. Then, all the rats were killed for examination of the LC content, sperm concentration, sperm motility and expression of OCTN2 mRNA in the epididymis and observation of the histopathological changes in the testis tissue.
Compared with the AZS model controls, the rats in the HSC and LC groups showed significantly increased LC content (2 880.3 vs 6 366.5 and 6 934.7 mg/L, P < 0.01), sperm concentration (［34.58 ± 10.25］ vs ［46.19 ± 14.23］ and ［42.25 ± 6.11］ ×10⁶/ml, P < 0.01), sperm motility (［42.59 ± 7.54］% vs ［61.34 ± 7.98］% and ［61.34 ± 7.98］%, P < 0.01) and expression of OCTN2 mRNA in the epididymis (26.07% vs 27.26% and 27.15%, P < 0.01). The animals of the HSC group exhibited a higher comparability than those of the LC group to the blank controls in the morphology, arrangement and activity of spermatogenic cells.
HSC can protect the reproductive function and improve sperm concentration and motility in the model rats with ornidazole-induced AZS, which may be associated with its abilities of up-regulating the expression of OCTN2 mRNA and increasing the LC content in the epididymis.
探讨环芍胶囊（HSC）对奥硝唑诱导的大鼠弱精子症（AZS）生殖功能保护作用的可能机制。
将40只SD雄性大鼠随机分为4组，每组数量相等，即空白对照组、AZS模型对照组、HSC干预组和L-肉碱（LC）干预组。后三组大鼠采用灌胃给予奥硝唑400mg/kg/d，连续28天建立AZS模型，同时HSC组和LC组大鼠分别给予HSC 0.31g/kg/d和LC 100mg/kg/d灌胃处理。然后，处死所有大鼠，检测附睾中LC含量、精子浓度、精子活力及OCTN2 mRNA表达，并观察睾丸组织的组织病理学变化。
与AZS模型对照组相比，HSC组和LC组大鼠附睾中LC含量显著升高（2880.3 vs 6366.5和6934.7mg/L，P<0.01），精子浓度显著升高（［34.58±10.25］vs［46.19±14.23］和［42.25±6.11］×10⁶/ml，P<0.01），精子活力显著升高（［42.59±7.54］% vs［61.34±7.98］%和［61.34±7.98］%，P<0.01），附睾中OCTN2 mRNA表达显著升高（26.07% vs 27.26%和27.15%，P<0.01）。HSC组大鼠生精细胞的形态、排列及活性与空白对照组相比，与LC组相比具有更高的可比性。
HSC可保护奥硝唑诱导的AZS模型大鼠的生殖功能，提高精子浓度和活力，这可能与其上调附睾中OCTN2 mRNA表达及增加LC含量的能力有关。
