Zhang Guo-Wei, Wan Xiu-Xia, Wan Chang-Chun, Li Kai-Qiang, Li Yi-Ze, Weng Zhi-Qiang, Shang Xue-Jun
Department of Andrology, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Virtual Laboratory Section, Laboratory of Human Integrated Biological Functions, School of Basic Medicine, Qingdao University, Qingdao, Shandong 266071, China.
Zhonghua Nan Ke Xue. 2018 Apr;24(4):297-303.
To study the protective effect of lipoic acid (LA) on the spermatogenic function of the male rats with oligoasthenozoospermia induced by ornidazole (ORN).
Seventy male SD rats were equally randomized into groups A (solvent control: 1 ml 0.5% CMC-Na + 1 ml olive oil), B (low-dose ORN model: 400 mg/kg ORN suspension + 1 ml olive oil), C (low-dose ORN + low-dose LA treatment: 400 mg/kg ORN + 50 mg/kg LA), D (low-dose ORN + high-dose LA treatment: 400 mg/kg ORN + 100 mg/kg LA), E (high-dose ORN model: 800 mg/kg ORN suspension + 1 ml olive oil), F (high-dose ORN + low-dose LA treatment: 800 mg/kg ORN + 50 mg/kg LA), and G (high-dose ORN + high-dose LA treatment: 800 mg/kg ORN + 100 mg/kg LA), and treated respectively for 20 successive days. Then all the rats were sacrificed and the weights of the body, testis, epididymis and seminal vesicle obtained, followed by calculation of the organ index, determination of epididymal sperm concentration and motility, and observation of the histomorphological changes in the testis and epididymis by HE staining.
Compared with group A, group E showed significantly decreased body weight (［117.67 ± 11.53］ vs ［88.11 ± 12.65］ g, P < 0.01) and indexes of the testis (［1.06 ± 0.12］ vs ［0.65 ± 0.13］ %, P < 0.01) and epididymis (［0.21 ± 0.03］ vs ［0.17 ± 0.01］ %, P < 0.01). In comparison with group E, group F exhibited remarkable increases in the epididymal index (［0.17 ± 0.01］ vs ［0.20 ± 0.02］ %, P < 0.01), and so did group G in the body weight (［88.11 ± 12.65］ vs ［102.70 ± 16.10］ g, P < 0.05) and the indexes of the testis (［0.65 ± 0.13］ vs ［0.95 ± 0.06］ %, P < 0.01) and epididymis (［0.17 ± 0.01］ vs ［0.19 ± 0.02］ %, P < 0.05), but no obvious difference was observed in the index of seminal vesicle among different groups. Compared with group A, group B manifested significant decreases in sperm motility (［74.12 ± 8.73］ vs ［40.25 ± 6.08］ %, P < 0.01), and so did group E in sperm count (［38.59 ± 6.40］ vs ［18.67 ± 4.59］ ×105/100 mg, P < 0.01) and sperm motility (［74.12 ± 8.73］ vs ［27.58 ± 8.43］ %, P < 0.01). Sperm motility was significantly lower in group B than in C and D (［40.25 ± 6.08］ vs ［58.13 ± 7.62］ and ［76.04 ± 8.44］%, P < 0.01), and so were sperm count and motility in group E than in F and G (［18.67 ± 4.59］ vs ［25.63 ± 9.66］ and ［29.92 ± 4.15］ ×105/100 mg, P < 0.05 and P < 0.01; ［27.58 ± 8.43］ vs ［36.56 ± 11.08］ and ［45.05 ± 9.59］ %, P < 0.05 and P < 0.01). There were no obvious changes in the histomorphology of the testis and epididymis in groups A, B, C and D. Compared with group A, group E showed necrotic and exfoliated spermatogenic cells with unclear layers and disorderly arrangement in the seminiferous tubules and remarkably reduced sperm count with lots of noncellular components in the epididymal cavity, while groups F and G exhibited increased sperm count in the seminiferous tubules and epididymis lumen, also with exfoliation, unclear layers and disorderly arrangement of spermatogenic cells, but significantly better than in group E.
LA can reduce ORN-induced damage to the spermatogenetic function of rats, improve sperm quality, and protect the reproductive system.
研究硫辛酸（LA）对奥硝唑（ORN）诱导的少弱精子症雄性大鼠生精功能的保护作用。
将70只雄性SD大鼠随机分为A组（溶剂对照组：1 ml 0.5%羧甲基纤维素钠+1 ml橄榄油）、B组（低剂量ORN模型组：400 mg/kg ORN混悬液+1 ml橄榄油）、C组（低剂量ORN+低剂量LA治疗组：400 mg/kg ORN+50 mg/kg LA）、D组（低剂量ORN+高剂量LA治疗组：400 mg/kg ORN+100 mg/kg LA）、E组（高剂量ORN模型组：800 mg/kg ORN混悬液+1 ml橄榄油）、F组（高剂量ORN+低剂量LA治疗组：800 mg/kg ORN+50 mg/kg LA）和G组（高剂量ORN+高剂量LA治疗组：800 mg/kg ORN+100 mg/kg LA），连续治疗20天。然后处死所有大鼠，称取体重、睾丸、附睾和精囊重量，计算器官指数，测定附睾精子浓度和活力，HE染色观察睾丸和附睾组织形态学变化。
与A组相比，E组体重（［117.67±11.53］ vs ［88.11±12.65］ g，P<0.01）、睾丸指数（［1.06±0.12］ vs ［0.65±0.13］%，P<0.01）和附睾指数（［0.21±0.03］ vs ［0.17±0.01］%，P<0.01）显著降低。与E组相比，F组附睾指数显著升高（［0.17±0.01］ vs ［0.20±0.02］%，P<0.01），G组体重（［88.11±12.65］ vs ［102.70±16.10］ g，P<0.05）、睾丸指数（［0.65±0.13］ vs ［0.95±0.06］%，P<0.01）和附睾指数（［0.17±0.01］ vs ［0.19±0.02］%，P<0.05）显著升高，不同组间精囊指数无明显差异。与A组相比，B组精子活力显著降低（［74.12±8.73］ vs ［40.25±6.08］%，P<0.01），E组精子计数（［38.59±6.40］ vs ［18.67±4.59］×105/100 mg，P<0.01）和精子活力（［74.12±8.73］ vs ［27.58±8.43］%，P<0.01）显著降低。B组精子活力显著低于C组和D组（［40.25±6.08］ vs ［58.13±7.62］和［76.04±8.44］%，P<0.01），E组精子计数和活力也显著低于F组和G组（［18.67±4.59］ vs ［25.63±9.66］和［29.92±4.15］×105/100 mg，P<0.05和P<0.01；［27.58±8.43］ vs ［36.56±11.08］和［4
