Katsumata Masaya, Kobayashi Hiroyuki, Ashihara Akane, Ishida Aiko
NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
Anim Sci J. 2018 Jul;89(7):988-993. doi: 10.1111/asj.13019. Epub 2018 Apr 29.
This study was conducted to test our hypothesis that intramuscular fat (IMF) accumulation increases in pigs fed on a low lysine diet during the dark period than those fed on the same diet during the light period. Using barrows aged 6 weeks, we monitored whether serum glucose and insulin levels were affected by light conditions. Two diets with different levels of lysine, 0.78% (LL diet) and 1.37% (control diet) were prepared. Eight pigs were fed on the diet during the light period, while the remaining pigs were fed during the dark period. The pigs were fed either the LL diet or the control diet. Although IMF contents of Longissimus dorsi (LD) muscle were higher in the pigs fed on a LL diet (p < .05), the light conditions had no effect. Low dietary lysine caused reduction in serum glucose levels (p < .05) and serum insulin levels (p = .0613). However, they were also unaffected by the lighting conditions. To gain further insights, we determined the messenger RNA levels of insulin receptor, insulin receptor substrate 1, acetyl CoA carboxylase, and fatty acid synthase in LD and Rhomboideus muscles and in the liver.
与在光照期饲喂相同日粮的猪相比，在黑暗期饲喂低赖氨酸日粮的猪肌肉内脂肪（IMF）积累增加。我们选用6周龄的公猪，监测光照条件是否会影响血清葡萄糖和胰岛素水平。制备了两种不同赖氨酸水平的日粮，分别为0.78%（低赖氨酸日粮，LL日粮）和1.37%（对照日粮）。8头猪在光照期饲喂日粮，其余猪在黑暗期饲喂。这些猪分别饲喂LL日粮或对照日粮。尽管饲喂LL日粮的猪背最长肌（LD）的IMF含量更高（p < 0.05），但光照条件并无影响。低日粮赖氨酸导致血清葡萄糖水平降低（p < 0.05）和血清胰岛素水平降低（p = 0.0613）。然而，它们也不受光照条件的影响。为了进一步深入了解，我们测定了LD肌、菱形肌和肝脏中胰岛素受体、胰岛素受体底物1、乙酰辅酶A羧化酶和脂肪酸合酶的信使核糖核酸水平。
