Department of Biological Sciences, University of Southern California, Los Angeles, CA, USA.
Department of Population Health and Reproduction, University of California Davis, Davis, CA, USA.
Environ Microbiol. 2018 Aug;20(8):2865-2879. doi: 10.1111/1462-2920.14259. Epub 2018 Jul 26.
A metatranscriptome study targeting the protistan community was conducted off the coast of Southern California, at the San Pedro Ocean Time-series station at the surface, 150 m (oxycline), and 890 m to link putative metabolic patterns to distinct protistan lineages. Comparison of relative transcript abundances revealed depth-related shifts in the nutritional modes of key taxonomic groups. Eukaryotic gene expression in the sunlit surface environment was dominated by phototrophs, such as diatoms and chlorophytes, and high abundances of transcripts associated with synthesis pathways (e.g., photosynthesis, carbon fixation, fatty acid synthesis). Sub-euphotic depths (150 and 890 m) exhibited strong contributions from dinoflagellates and ciliates, and were characterized by transcripts relating to digestion or intracellular nutrient recycling (e.g., breakdown of fatty acids and V-type ATPases). These transcriptional patterns underlie the distinct nutritional modes of ecologically important protistan lineages that drive marine food webs, and provide a framework to investigate trophic dynamics across diverse protistan communities.
本研究针对原生生物群落进行了宏转录组学研究，地点位于美国南加州海岸的圣佩德罗海洋时间序列站，采样深度分别为表层、150 米（氧化层）和 890 米，旨在将假定的代谢模式与不同的原生生物类群联系起来。相对转录丰度的比较揭示了关键分类群营养模式与深度的相关性变化。在阳光充足的表层环境中，真核生物的基因表达主要由硅藻和绿藻等光养生物主导，与合成途径相关的高丰度转录本（如光合作用、碳固定、脂肪酸合成）。亚透光层（150 米和 890 米）显示出甲藻和纤毛虫的强烈贡献，并以与消化或细胞内营养回收相关的转录本为特征（例如脂肪酸和 V 型 ATP 酶的分解）。这些转录模式是驱动海洋食物网的生态重要原生生物类群的独特营养模式的基础，并为研究不同原生生物群落的营养动态提供了框架。
