Gaffney E V, Koch G, Tsai S C, Loucks T, Lingenfelter S E
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cancer Res. 1988 Oct 1;48(19):5455-9.
Human recombinant interleukin 1 alpha (IL-1 alpha) and IL-1 beta inhibited the replication of the mammary tumor cell line, MDA-MB-415; stimulated division in the colon carcinoma, SW-48; and had no effect on the growth of the milk mammary line, HBL-100. Inhibition of growth was reflected in a significant decrease in DNA synthesis accompanying a transient increase in RNA synthesis. Specific binding of 125I-labeled recombinant IL-1 beta by MDA-MB-415 and SW-48 reached a maximum by 2 h of incubation, and an equivalent amount was bound by each cell type. Binding was inhibited in a dose-dependent manner by unlabeled IL-1 alpha or IL-1 beta. Scatchard plot analysis revealed that MDA-MB-415 cells expressed approximately 700 binding sites with an apparent dissociation constant of 8.8 x 10(-10) M. Reversibility of growth inhibition was independent of dose or time of incubation, but DNA synthesis did not return to control values. Flow cytometric analysis of DNA content showed that growth inhibition was cell cycle phase nonspecific with a slight reduction in the proportion of cells in S phase. The major conclusion from these studies was that inhibition or stimulation of malignant cell growth by IL-1 was related to the presence of receptor sites.
人重组白细胞介素1α（IL-1α）和IL-1β抑制乳腺肿瘤细胞系MDA-MB-415的复制；刺激结肠癌细胞系SW-48的分裂；对乳腺细胞系HBL-100的生长无影响。生长抑制表现为DNA合成显著减少，同时RNA合成短暂增加。MDA-MB-415和SW-48对125I标记的重组IL-1β的特异性结合在孵育2小时时达到最大值，且每种细胞类型结合的量相当。未标记的IL-1α或IL-1β以剂量依赖的方式抑制结合。Scatchard图分析显示，MDA-MB-415细胞表达约700个结合位点，表观解离常数为8.8×10-10 M。生长抑制的可逆性与孵育剂量或时间无关，但DNA合成未恢复到对照值。DNA含量的流式细胞术分析表明，生长抑制是非细胞周期阶段特异性的，S期细胞比例略有降低。这些研究的主要结论是，IL-1对恶性细胞生长的抑制或刺激与受体位点的存在有关。
