Agliassa Chiara, Narayana Ravishankar, Bertea Cinzia M, Rodgers Christopher T, Maffei Massimo E
Plant Physiology Unit, Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy.
Department of Entomology, University Park, Pennsylvania.
Bioelectromagnetics. 2018 Jul;39(5):361-374. doi: 10.1002/bem.22123. Epub 2018 Apr 30.
Variations in magnetic field (MF) intensity are known to induce plant morphological and gene expression changes. In Arabidopsis thaliana Col-0, near-null magnetic field (NNMF, i.e., <100 nT MF) causes a delay in the transition to flowering, but the expression of genes involved in this response has been poorly studied. Here, we showed a time-course quantitative analysis of the expression of both leaf (including clock genes, photoperiod pathway, GA20ox, SVP, and vernalization pathway) and floral meristem (including GA2ox, SOC1, AGL24, LFY, AP1, FD, and FLC) genes involved in the transition to flowering in A. thaliana under NNMF. NNMF induced a delayed flowering time and a significant reduction of leaf area index and flowering stem length, with respect to controls under geomagnetic field. Generation experiments (F - and F -NNMF) showed retention of flowering delay. The quantitative expression (qPCR) of some A. thaliana genes expressed in leaves and floral meristem was studied during transition to flowering. In leaves and flowering meristem, NNMF caused an early downregulation of clock, photoperiod, gibberellin, and vernalization pathways and a later downregulation of TSF, AP1, and FLC. In the floral meristem, the downregulation of AP1, AGL24, FT, and FLC in early phases of floral development was accompanied by a downregulation of the gibberellin pathway. The progressive upregulation of AGL24 and AP1 was also correlated to the delayed flowering by NNMF. The flowering delay is associated with the strong downregulation of FT, FLC, and GA20ox in the floral meristem and FT, TSF, FLC, and GA20ox in leaves. Bioelectromagnetics. 39:361-374, 2018. © 2018 The Authors. Bioelectromagnetics Published by Wiley Periodicals, Inc.
已知磁场（MF）强度的变化会诱导植物形态和基因表达的改变。在拟南芥Col-0中，近零磁场（NNMF，即<100 nT MF）会导致开花转变延迟，但参与此反应的基因表达研究较少。在此，我们展示了在NNMF条件下，拟南芥中参与开花转变的叶片（包括生物钟基因、光周期途径、GA20ox、SVP和春化途径）和花分生组织（包括GA2ox、SOC1、AGL24、LFY、AP1、FD和FLC）基因表达的时间进程定量分析。与地磁场条件下的对照相比，NNMF导致开花时间延迟，叶面积指数和花茎长度显著降低。代际实验（F - 和F -NNMF）表明开花延迟得以保留。在开花转变过程中，研究了一些在叶片和花分生组织中表达的拟南芥基因的定量表达（qPCR）。在叶片和花分生组织中，NNMF导致生物钟、光周期、赤霉素和春化途径早期下调，以及TSF、AP1和FLC后期下调。在花分生组织中，花发育早期AP1、AGL24、FT和FLC的下调伴随着赤霉素途径的下调。AGL24和AP1的逐渐上调也与NNMF导致的开花延迟相关。开花延迟与花分生组织中FT、FLC和GA20ox以及叶片中FT、TSF、FLC和GA20ox的强烈下调有关。生物电磁学。39:361 - 374，2018。©2018作者。生物电磁学由威利期刊公司出版。
