Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China; Northeastern University, 3-11 Wenhua Road, Shenyang 110819, China.
Department of Stomatology, General Hospital of Shenyang Military Area, Shenyang 110016, China.
Dent Mater. 2018 Aug;34(8):1112-1126. doi: 10.1016/j.dental.2018.04.007. Epub 2018 Apr 27.
A novel copper-bearing titanium alloy (Ti-Cu) was fabricated for dental application that is expected to efficiently restrain the growth of bacteria and discourage biofilm formation. The aim of this study was to investigate both the antibacterial activity and biofilm inhibition of Ti-Cu alloy in vitro, and the antibacterial effect of Ti-Cu implant in early stage of peri-implantitis in vivo.
Staphylococcus aureus and Escherichia coli were selected to evaluate the antibacterial activity of Ti-Cu alloy and Ti served as control. The antibacterial rate, attached bacteria and developed biofilms were studied from quantitative antibacterial test, biofilm observation and bacterial morphological examination. Electrochemical tests were used to investigate the corrosion property of Ti-Cu alloy. Furthermore, both Ti and Ti-Cu dental implants were manufactured and then implanted in the mandibular premolar sites of beagle dogs for 3 months with ligature-infected treatment. Implant-tissue samples were prepared for radiographic analysis, Micro-CT evaluation and histological examination.
Ti-Cu alloy was found to efficiently kill the attached bacteria by ways of damaging cell membranes and cell walls and strongly inhibit the biofilm formation. However, Ti-Cu alloy had excellent corrosion resistance similar with Ti. Further, Ti-Cu dental implants showed superior capacities of inhibiting the bone resorption caused by bacterial infection and enhancing bone formation.
Ti-Cu alloy strongly inhibited biofilm formation in vitro and prevented bacterial infection associated with dental implant in vivo, making it great potential for application in dental implants with excellent antibacterial viability and positive effect against bone resorption induced by peri-implantitis.
开发一种新型含铜钛合金（Ti-Cu），用于口腔医学，以有效抑制细菌生长和阻止生物膜形成。本研究旨在体外研究 Ti-Cu 合金的抗菌活性和生物膜抑制作用，以及 Ti-Cu 种植体在早期种植体周围炎中的体内抗菌效果。
选择金黄色葡萄球菌和大肠杆菌评估 Ti-Cu 合金的抗菌活性，以 Ti 作为对照。通过定量抗菌试验、生物膜观察和细菌形态学检查研究抗菌率、附着细菌和形成的生物膜。电化学测试用于研究 Ti-Cu 合金的腐蚀性能。此外，制造 Ti 和 Ti-Cu 牙科植入物，然后用结扎感染处理将其植入比格犬下颌前磨牙部位 3 个月。准备植入物-组织样本进行放射学分析、Micro-CT 评估和组织学检查。
Ti-Cu 合金通过破坏细胞膜和细胞壁有效地杀死附着的细菌，并强烈抑制生物膜的形成。然而，Ti-Cu 合金具有与 Ti 相似的优异耐腐蚀性。此外，Ti-Cu 牙科植入物具有抑制细菌感染引起的骨吸收和增强骨形成的卓越能力。
Ti-Cu 合金在体外强烈抑制生物膜形成，并在体内防止与牙科植入物相关的细菌感染，使其具有在具有优异抗菌活力和对种植体周围炎引起的骨吸收有积极作用的牙科植入物中应用的巨大潜力。
