Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran; Faculty of Materials and Chemical Engineering, Shahrood University of Technology, P. O. Box: 3619995161, Semnan, Iran.
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran.
Int J Biol Macromol. 2018 Sep;116:364-377. doi: 10.1016/j.ijbiomac.2018.04.137. Epub 2018 Apr 30.
The main aim of this study was to evaluate the suitability of sulfonated alginate as a modifying agent to enhance the hemocompatibility of self-fabricated polyethersulfone (PES) hollow fiber membrane for blood detoxification. Sodium alginate was sulfonated with a degree of 0.6 and immobilized on the membrane via surface amination and using glutaraldehyde as cross-linking agent. Coating layer not only improved the membrane surface hydrophilicity, but also induced -39.2 mV negative charges on the surface. Water permeability of the modified membrane was enhanced from 67 to 95 L/m·h·bar and flux recovery ratio increased more than 2-fold. Furthermore, the modified membrane presented higher platelet adhesion resistance (reduced by more than 90%) and prolonged coagulation time (35 s for APTT and 14 s for PT) in comparison with the pristine PES hollow fiber membrane, which verified the improved anti-thrombogenicity of the modified membrane. On the other hand, obtained membrane after 3 h coating could remove up-to 60% of the uremic toxins. According to the obtained data, sulfonated alginate can be a promising modifying agent for the future blood-contacting membrane and specially blood purification issues.
本研究的主要目的是评估磺化海藻酸钠作为一种修饰剂的适用性，以增强自制聚醚砜（PES）中空纤维膜的血液解毒血液相容性。用 0.6 的程度对海藻酸钠进行磺化，并通过表面氨化将其固定在膜上，同时使用戊二醛作为交联剂。涂层不仅提高了膜表面的亲水性，而且在表面诱导出-39.2 mV 的负电荷。改性膜的水通量从 67 增加到 95 L/m·h·bar，通量恢复率增加了两倍以上。此外，与原始 PES 中空纤维膜相比，改性膜具有更高的血小板粘附阻力（降低超过 90%）和更长的凝血时间（APTT 为 35 s，PT 为 14 s），这证明了改性膜的抗血栓形成性得到了改善。另一方面，经过 3 小时涂层后，获得的膜可以去除高达 60%的尿毒症毒素。根据获得的数据，磺化海藻酸钠可以成为未来用于血液接触膜和专门用于血液净化问题的有前途的修饰剂。
