Graduate School of Bioagricultural Science, Nagoya University, Nagoya, 464-8602 Aichi, Japan.
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602 Aichi, Japan.
Proc Natl Acad Sci U S A. 2018 May 15;115(20):E4710-E4719. doi: 10.1073/pnas.1804233115. Epub 2018 Apr 30.
Reactive oxygen species (ROS) are known to be important signal molecules that are involved in biotic and abiotic stress responses as well as in growth regulation. However, the molecular mechanisms by which ROS act as a growth regulator, as well as how ROS-dependent growth regulation relates to its roles in stress responses, are not well understood. We performed a time-course microarray analysis of root tips upon treatment with hydrogen peroxide, which we named "ROS-map." Using the ROS-map, we identified an MYB transcription factor, MYB30, which showed a strong response to ROS treatment and is the key regulator of a gene network that leads to the hydrogen peroxide-dependent inhibition of root cell elongation. Intriguingly, this network contained multiple genes involved in very-long-chain fatty acid (VLCFA) transport. Finally, we showed that is necessary for root growth regulation during defense responses, thus providing a molecular link between these two ROS-associated processes.
活性氧（ROS）是一种重要的信号分子，参与生物和非生物胁迫反应以及生长调控。然而，ROS 作为生长调节剂的分子机制，以及 ROS 依赖性生长调控与其在胁迫反应中的作用之间的关系，尚不清楚。我们对根尖端进行了过氧化氢处理的时间进程微阵列分析，我们将其命名为“ROS-map”。利用 ROS-map，我们鉴定出一个 MYB 转录因子 MYB30，它对 ROS 处理有强烈反应，是导致过氧化氢依赖性抑制根细胞伸长的基因网络的关键调节因子。有趣的是，这个网络包含多个参与超长链脂肪酸（VLCFA）运输的基因。最后，我们表明在防御反应中，对于根生长调控是必需的，因此为这两个与 ROS 相关的过程之间提供了一个分子联系。
