Nurkhasanah -, Sulistyani Nanik, Fatmawati Ery
Facullty of Pharmacy, Universitas Ahmad Dahlan, Jl Prof. Soepomo, Janturan, Yogyakarta, Indonesia.
Facullty of Pharmacy, Universitas Ahmad Dahlan, Jl Prof. Soepomo, Janturan, Yogyakarta, Indonesia / STIKES Madani, Jl. Wonosari Km.10 Karanggayam, Sitimulyo, Piyungan, Bantul, Yogyakarta, Indonesia.
Pak J Pharm Sci. 2018 May;31(3):849-856.
The objective of the present study was to characterize the activity and expression of catalase enzymes in Hibiscus sabdariffa L (rosella) extract treated rat induced by dimethylbenz-α-anthracene (DMBA) and to evaluate the relationship between the catalase activity and histopathological characteristics of liver organ. The 25 animals were divided randomly into 5 groups: the normal group, the negative control group, and treated groups, which treated by rosella extract with variation of dose of 10, 50 and 100mg/kgBW/day for 35 days. On day36 the animals were given with DMBA in dose of 75 mg/kgBW single dose. After one week, the animals were sacrificed and the catalase activity was measured from liver homogenate by the decomposition of HO and followed directly by the decrease in absorbance at 240 nm. The expression of catalase gene was observed using RT-PCR. The results showed that treatment of rosella extract increases the activity of catalase, significantly (P<0.05). The increasing of catalase activity was found in dose dependent manner. The catalase gene expression was also found to increase in rosella extract treated groups. The histopathological observation of liver organ was found normal. Rosella extract increase the catalase activity and expression of catalase antioxidant gene. It was concluded that rosella extract increase activity and gene expression of catalase in vivo.
本研究的目的是表征经玫瑰茄提取物处理的、由二甲基苯并-α-蒽（DMBA）诱导的大鼠中过氧化氢酶的活性和表达，并评估过氧化氢酶活性与肝脏器官组织病理学特征之间的关系。将25只动物随机分为5组：正常组、阴性对照组和处理组，处理组分别用剂量为10、50和100mg/kg体重/天的玫瑰茄提取物处理35天。在第36天，给动物单次注射剂量为75mg/kg体重的DMBA。一周后，处死动物，通过HO的分解并直接跟踪240nm处吸光度的降低，从肝脏匀浆中测量过氧化氢酶活性。使用RT-PCR观察过氧化氢酶基因的表达。结果表明，玫瑰茄提取物处理可显著提高过氧化氢酶的活性（P<0.05）。过氧化氢酶活性的增加呈剂量依赖性。在玫瑰茄提取物处理组中也发现过氧化氢酶基因表达增加。肝脏器官的组织病理学观察正常。玫瑰茄提取物增加了过氧化氢酶的活性和过氧化氢酶抗氧化基因的表达。得出的结论是，玫瑰茄提取物在体内增加了过氧化氢酶的活性和基因表达。
