Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Department of Biochemistry and Biophysics, Tokyo Medical and Dental University, Tokyo, Japan.
Stem Cell Res Ther. 2018 May 2;9(1):123. doi: 10.1186/s13287-018-0870-9.
Synovial mesenchymal stem cells (MSCs) are an attractive cell source for cartilage and meniscus regeneration. Synovial tissue can be histologically classified into three regions; surface, stromal and perivascular region, but the localization of synovial MSCs has not been fully investigated. We identified markers specific for each region, and compared properties of MSCs derived from each region in the synovium.
The intensity of immunostaining with 19 antibodies was examined for surface, stromal, and perivascular regions of human synovium from six osteoarthritis patients. Specific markers were identified and synovial cells derived from each region were sorted. Proliferation, surface marker expression, chondrogenesis, calcification and adipogenesis potentials were compared in synovial MSCs derived from the three regions.
We selected CD55 CD271 for synovial cells in the surface region, CD55 CD271 in the stromal region, and CD55 CD271 in the perivascular region. The ratio of the sorted cells to non-hematopoietic lineage cells was 5% in the surface region, 70% in the stromal region and 15% in the perivascular region. Synovial cells in the perivascular fraction had the greatest proliferation potential. After expansion, surface marker expression profiles and adipogenesis potentials were similar but chondrogenic and calcification potentials were higher in synovial MSCs derived from the perivascular region than in those derived from the surface and stromal regions.
We identified specific markers to isolate synovial cells from the surface, stromal, and perivascular regions of the synovium. Synovial MSCs in the perivascular region had the highest proliferative and chondrogenic potentials among the three regions.
滑膜间充质干细胞（MSCs）是软骨和半月板再生的有吸引力的细胞来源。滑膜组织可以在组织学上分为三个区域：表面、基质和血管周区域，但滑膜 MSCs 的定位尚未得到充分研究。我们鉴定了每个区域特有的标志物，并比较了滑膜中每个区域来源的 MSCs 的特性。
对来自 6 名骨关节炎患者的滑膜的表面、基质和血管周区域的 19 种抗体的免疫染色强度进行了检查。鉴定了特定的标志物，并对来自每个区域的滑膜细胞进行了分类。比较了源自三个区域的滑膜 MSCs 的增殖、表面标志物表达、软骨形成、钙化和脂肪形成潜能。
我们选择 CD55 CD271 作为表面区域的滑膜细胞、CD55 CD271 作为基质区域的滑膜细胞，以及 CD55 CD271 作为血管周区域的滑膜细胞。在表面区域，分选细胞与非造血谱系细胞的比例为 5%，在基质区域为 70%，在血管周区域为 15%。血管周部分的滑膜细胞具有最大的增殖潜力。扩增后，表面标志物表达谱和脂肪形成潜能相似，但源自血管周区域的滑膜 MSC 的软骨形成和钙化潜能高于源自表面和基质区域的滑膜 MSC。
我们鉴定了特定的标志物，用于从滑膜的表面、基质和血管周区域分离滑膜细胞。在三个区域中，血管周区域的滑膜 MSC 具有最高的增殖和软骨形成潜能。
