Section of Oral and Maxillofacial Surgery, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, Verona, Italy.
J Biol Regul Homeost Agents. 2018 Mar-Apr;32(2 Suppl. 2):75-80.
Metal ions accumulate inside the epithelial cells of the oral mucosa and damage the DNA. The aim of this study was to analyze whether DNA damage and/or apoptosis also occurs after a short-term exposure to the metals of fixed orthodontic appliances. 23 subjects were enrolled in the test group and 8 patients in the control group. Analyses performed on samples of oral mucosa were cell count, cellular viability, quantification of intracellular metal concentration, comet and micronuclei tests and quantification of intracellular Glutathione (GSH). The obtained results indicated that orthodontic appliances release metal ions, especially chromium, that cause inflammatory processes leading to DNA damages. These phenomenon are visible only after 30 days from application of fixed orthodontic appliances.
金属离子在口腔黏膜上皮细胞内蓄积并损伤 DNA。本研究旨在分析固定正畸矫治器中的金属在短期暴露后是否也会导致 DNA 损伤和/或细胞凋亡。23 名受试者被纳入实验组，8 名患者被纳入对照组。对口腔黏膜样本进行细胞计数、细胞活力分析、细胞内金属浓度定量、彗星和微核试验以及细胞内谷胱甘肽 (GSH) 定量分析。结果表明，正畸矫治器释放金属离子，特别是铬，引起炎症反应导致 DNA 损伤。这些现象仅在固定正畸矫治器应用 30 天后才可见。
