Atallah Nadia M, Vitek Olga, Gaiti Federico, Tanurdzic Milos, Banks Jo Ann
Purdue University Center for Cancer Research.
College of Computer and Information Science, Northeastern University, Boston, MA 02115.
G3 (Bethesda). 2018 Jul 2;8(7):2205-2214. doi: 10.1534/g3.118.200292.
The fern is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants.
蕨类植物是研究同型孢子植物性别决定和配子分化的重要模式植物。在这里，我们使用RNA测序来组装转录组，并鉴定在幼配子体中差异表达的基因，因为它们的性别由称为雄器素的雄性诱导信息素的存在与否决定。在鉴定出的1163个共有差异表达基因中，绝大多数（1030个）在经雄器素处理的配子体中上调。对这些差异表达基因的基因本体论（GO）术语富集分析表明，参与配子体基因组表观遗传重编程的大量基因被该信息素上调。其他激素反应和发育基因也被该信息素上调。这个配子体转录组和基因表达数据集将被证明对专注于植物性别决定和分化的研究有用。
