Kamachi Hiroyuki, Iwasawa Orie, Hickok Leslie G, Nakayama Masaaki, Noguchi Munenori, Inoue Hiroshi
Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
J Plant Res. 2007 Sep;120(5):629-34. doi: 10.1007/s10265-007-0106-z. Epub 2007 Aug 3.
The sexuality of homosporous fern gametophytes is usually determined by antheridiogen, a pheromone that promotes maleness. In this work the effect of photomorphogenically active light on antheridiogen-induced male development was examined for gametophytes of Ceratopteris richardii. Although blue light did not affect sensitivity to Ceratopteris antheridiogen (A(Ce)) in wild-type gametophytes, it was found that the gametophytes of the her1 mutant, which are insensitive to A(Ce), developed into males when grown under blue light in the presence of A(Ce). Thus, we conclude that another A(Ce)-signal transduction pathway activated by blue light exists latently in the gametophytes of C. richardii. Red light, on the other hand, suppressed male development. Because simultaneous red and blue light-irradiation did not promote male development in the her1 gametophytes, the action of red light seems to dominate that of blue light. The results of experiments with a photomorphogenic mutant also suggested that phytochrome may be involved in the action of red light.
同型孢子蕨类配子体的性别通常由成雄素决定，成雄素是一种促进雄性特征的信息素。在这项研究中，针对里氏水龙骨的配子体，研究了光形态建成活性光对成雄素诱导的雄性发育的影响。虽然蓝光不会影响野生型配子体对里氏水龙骨成雄素（A(Ce)）的敏感性，但研究发现，对A(Ce)不敏感的her1突变体配子体，在蓝光下且存在A(Ce)的条件下生长时会发育为雄性。因此，我们得出结论，蓝光激活的另一条A(Ce)信号转导途径潜在地存在于里氏水龙骨的配子体中。另一方面，红光抑制雄性发育。由于同时进行红光和蓝光照射并未促进her1配子体的雄性发育，红光的作用似乎占主导地位。对光形态建成突变体的实验结果也表明，光敏色素可能参与了红光的作用。
