酵母 snoRNA 的核命运由共转录的 Rnt1 切割决定。
Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage.
机构信息
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
出版信息
Nat Commun. 2018 May 3;9(1):1783. doi: 10.1038/s41467-018-04094-y.
Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5'end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the mG cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3'end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5'end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5'end processing of box C/D snoRNA defines their distinct pathway of maturation.
小核仁 RNA（snoRNA）是保守且必需的非编码 RNA，由 RNA 聚合酶 II（Pol II）转录。两类 snoRNA，以前因其结构和核糖核蛋白组成而区分，作为引导 RNA 靶向如核糖体 RNA，从而引入特定的修饰。我们研究了单独转录的 snoRNA 在 S. cerevisiae 中的 5'端加工，以定义它们在 snoRNA 生物发生和功能中的作用。在这里，我们表明内切核酸酶 Rnt1 的 pre-snoRNA 加工与 mG 帽的去除是共转录的，这有利于框 C/D snoRNA 的形成。这个过程的失败会导致异常的 3'端加工和 snoRNA 错误定位到细胞质中。因此，Rnt1 依赖性的 box C/D snoRNA 的 5'端加工对于 snoRNA 依赖性的核糖体 RNA 甲基化至关重要。我们的结果表明，box C/D snoRNA 的 5'端加工定义了它们独特的成熟途径。
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