Department of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Gdańsk, Poland.
Sci Rep. 2018 May 3;8(1):6980. doi: 10.1038/s41598-018-25428-2.
Low-invasive in vivo solid-phase microextraction (SPME) was used to investigate the lipid profiles of muscle tissue of living fish. Briefly, mixed mode SPME fibers were inserted into the muscle for 20 min extraction, and then the fibers were desorbed in an optimal mixture of solvents. The obtained lipid profile was then compared and contrasted to that obtained with employment of ex vivo SPME and solid-liquid extraction (SLE) from fish muscle tissue belonging to the same group of fish, following a one-year storage period. Ex vivo SPME analysis of stored muscle samples revealed 10-fold decrease in the number of detected molecular features in comparison to in vivo study. Moreover, in vivo microsampling enabled the identification of different classes of bioactive lipids, including fatty acyls, not present in the lipid profile obtained through ex vivo SPME and SLE, suggesting the alterations occurring in the unbound lipid fraction of the system under study during the storage and also indicating the advantage of the in vivo extraction approach.
采用低侵入式活体固相微萃取（SPME）技术研究活体鱼类肌肉组织的脂质图谱。简要地说，混合模式 SPME 纤维插入肌肉中进行 20 分钟的萃取，然后将纤维在最佳溶剂混合物中解吸。然后将获得的脂质图谱与使用来自同一批鱼的鱼肌肉组织的离体 SPME 和固液萃取（SLE）获得的脂质图谱进行比较和对比，经过一年的储存期。与体内研究相比，储存肌肉样品的离体 SPME 分析显示，检测到的分子特征数量减少了 10 倍。此外，体内微采样能够鉴定不同类别的生物活性脂质，包括脂肪酸酯，这在通过离体 SPME 和 SLE 获得的脂质图谱中不存在，这表明在储存过程中研究体系中未结合脂质部分发生了变化，也表明了体内提取方法的优势。
