Gude M, Hausen B M, Heitsch H, König W A
Department of Dermatology, University Hospital, Hamburg, FRG.
Contact Dermatitis. 1988 Jul;19(1):1-10. doi: 10.1111/j.1600-0536.1988.tb02860.x.
Irritancy of daffodil flowers and bulbs was assessed using various fresh plant preparations, solvent extracts and some of the known Amaryllidaceae alkaloids on guinea pigs. Sensitization was also carried out on guinea pigs using these plant preparations, solvent extracts and 7 fractions obtained after preparative chromatography of the bulb ether extract. Only 1 fraction, containing 2 alkaloids, was capable of inducing delayed hypersensitivity in the animals; the sensitivity achieved, however, was weak. The substances were identified as masonin and homolycorin, which acted as elicitors, but masonin may also be a sensitizer. While homolycorin is a known daffodil constituent, masonin has not been found previously in Narcissus pseudonarcissus. 3 other alkaloids as well as chelidonic acid and isorhamnetin were non-elicitors in the sensitized guinea pigs.
使用各种新鲜植物制剂、溶剂提取物以及一些已知的石蒜科生物碱对豚鼠进行了水仙花和鳞茎的刺激性评估。还使用这些植物制剂、溶剂提取物以及鳞茎乙醚提取物经制备色谱法得到的7个馏分对豚鼠进行了致敏试验。只有1个含有2种生物碱的馏分能够在动物中诱导迟发型超敏反应；然而，所达到的敏感性较弱。这些物质被鉴定为石蒜碱和高石蒜碱，它们起激发剂的作用，但石蒜碱也可能是一种致敏剂。虽然高石蒜碱是水仙花的已知成分，但石蒜碱此前尚未在洋水仙中发现。另外3种生物碱以及白屈菜酸和异鼠李素在致敏豚鼠中不是激发剂。
