Horkay Ferenc, Basser Peter J, Hecht Anne-Marie, Geissler Erik
Section on Quantitative Imaging and Tissue Sciences, Eunice Kennedy Schriver National Institute of Child Health and Human Development, National Institutes of Health, 13 South Drive, Bethesda, MD 20892, USA.
Laboratoire de Spectrométrie Physique CNRS UMR 5588, Université J. Fourier de Grenoble, B.P.87, 38402 St Martin d'Hères cedex, France.
Macromol Symp. 2017 Apr;372(1):43-50. doi: 10.1002/masy.201700014. Epub 2017 Apr 19.
The most abundant cartilage proteoglycan is aggrecan, a bottlebrush shaped molecule that possesses over 100 glycosaminoglycan (chondroitin sulfate and keratan sulfate) chains. The side-chains are linear sulfated polysaccharides that are negatively charged under physiological conditions. Aggrecan interacts with hyaluronic acid (HA) to form large aggregates. Osmotic pressure measurements and rheological measurements are used to study the static and dynamic behavior of aggrecan assemblies at the macroscopic length scales. The microscopic properties of aggrecan solutions are determined by small angle neutron scattering (SANS), and static and dynamic light scattering (SLS and DLS). In dilute solutions aggrecan forms microgels with a diffuse boundary, composed of loosely connected clusters. The osmotic pressure of the aggrecan-HA system decreases with increasing HA content. DLS yields a relaxation rate that varies as , arising from internal modes in the microgel. The relaxation rate in the solutions of the aggrecan-HA complex is slightly greater than in the pure aggrecan solution.
最丰富的软骨蛋白聚糖是聚集蛋白聚糖，它是一种瓶刷状分子，拥有超过100条糖胺聚糖（硫酸软骨素和硫酸角质素）链。这些侧链是线性硫酸化多糖，在生理条件下带负电荷。聚集蛋白聚糖与透明质酸（HA）相互作用形成大的聚集体。渗透压测量和流变学测量用于研究聚集蛋白聚糖组装体在宏观长度尺度下的静态和动态行为。聚集蛋白聚糖溶液的微观性质由小角中子散射（SANS）以及静态和动态光散射（SLS和DLS）确定。在稀溶液中，聚集蛋白聚糖形成具有扩散边界的微凝胶，由松散连接的簇组成。聚集蛋白聚糖-HA系统的渗透压随HA含量增加而降低。动态光散射产生的弛豫速率随 变化，这源于微凝胶中的内部模式。聚集蛋白聚糖-HA复合物溶液中的弛豫速率略大于纯聚集蛋白聚糖溶液中的弛豫速率。
