Praharaj Manas R, Sahoo Aditya P, Chauhan Tapan K S, Gandham Ravi Kumar, Saxena Shikha, Agarwal Ravi K, Dhama Kuldeep, Mishra Bina, Marriappan Asok K, Tiwari Ashok K, Goswami Puroshottam Prasad, Mishra Bishnu Prasad, Kumar Deepak
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
ICAR- Directorate on Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand, India.
Oncotarget. 2018 Apr 13;9(28):19569-19583. doi: 10.18632/oncotarget.24668.
Avian reoviruses, members of genus was known to cause diseases like tenosynovitis, runting-stunting syndrome in chickens. Among eight structural proteins, the proteins of S-class are mainly associated with viral arthritis but the significance of σB protein in arthritis is not established till date. In this infection pathological condition together with infection of joints often leads to arthritis because joints consists of cartilage which forms lubricating surface between two bones, and has limited metabolic, replicative and repair capacity. To establish the role of σB protein in arthritis, an microarray study was conducted consisting four groups viz. virus infected and control; pDsRed-Express-N1-σB and empty pDs-Red transfected, CEF cells. With cut-off value as FC ≥2, p value <0.05, 6709 and 4026 numbers of DEGs in virus and σB, respectively were identified. The Ingenuity Pathway Analysis gave an idea about the involvement of σB protein in "osteoarthritis pathway", which was activated with z-score with 3.151. The pathway "Role of IL-17A in arthritis pathway" was also enriched with -log (p-value) 1.64. Among total 122 genes involved in osteoarthritis pathway, 28 upregulated and 11 downregulated DEGs were common to both virus and σB treated cells. Moreover, 14 upregulated and 7 downregulated were unique in σB transfected cells. Using qRT-PCR for IL-1B, BMP2, SMAD1, SPP1 genes, the microarray data was validated. We concluded that during ARV infection σB protein, if not fully partially leads to molecular alteration of various genes of host orchestrating the different molecular pattern in joints, leading to tenosynovitis syndrome.
禽呼肠孤病毒是呼肠孤病毒属的成员，已知可引起鸡的腱鞘炎、矮小综合征等疾病。在八种结构蛋白中，S类蛋白主要与病毒性关节炎有关，但迄今为止，σB蛋白在关节炎中的意义尚未明确。在这种感染的病理状态下，关节感染常导致关节炎，因为关节由软骨组成，软骨在两块骨头之间形成润滑表面，且代谢、复制和修复能力有限。为了确定σB蛋白在关节炎中的作用，进行了一项微阵列研究，包括四组，即病毒感染组和对照组；转染pDsRed-Express-N1-σB和空pDs-Red的鸡胚成纤维细胞（CEF）。以FC≥2、p值<0.05为截断值，分别在病毒组和σB组中鉴定出6709个和4026个差异表达基因（DEG）。 Ingenuity通路分析表明σB蛋白参与了“骨关节炎通路”，该通路的z值为3.151时被激活。“IL-17A在关节炎通路中的作用”通路也以-log（p值）1.64富集。在参与骨关节炎通路的总共122个基因中，病毒组和σB处理组细胞共有28个上调和11个下调的DEG。此外，在σB转染细胞中有14个上调和7个下调的基因是独特的。使用qRT-PCR检测IL-1B、BMP2、SMAD1、SPP1基因，验证了微阵列数据。我们得出结论，在禽呼肠孤病毒感染期间，σB蛋白即使不是完全也至少部分导致宿主各种基因的分子改变，从而在关节中协调不同的分子模式，导致腱鞘炎综合征。
