Wan Junjie, Wen Xintian, Huang Xiaobo, Tang Ying, Huang Yong, Yan Qigui, Zhao Qin, Cao Sanjie
Laboratory of Animal Infectious Disease and Microarray, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Vet Immunol Immunopathol. 2012 Jun 30;147(3-4):154-60. doi: 10.1016/j.vetimm.2012.04.009. Epub 2012 Apr 23.
Avian reovirus (ARV) is an important pathogen in poultry industry and causes great economic losses. As attenuated Salmonella typhimurium is already being used as an effective vehicle for the transfer of DNA vaccines, so in this study we evaluated two DNA vaccines mediated by S. typhimurium on their ability of eliciting antibody production. SPF chickens were respectively immunized with SL7207 (pVAX-σB), SL7207 (pVAX-σC) and SL7027 (pVAX-σB-σC) three times. The results showed that the antibody production was highly dependent on the immunizing times, detectable antibodies of serum antibody IgG and small intestinal mucosal antibody IgA were generated at week 4 and were further improved at week 6 and antibody titers in group SL7207 (pVAX-σC) were higher than that in group SL7207 (pVAX-σB), demonstrating that SL7207 (pVAX-σC) was more powerful than SL7207 (pVAX-σB) in antibody production. The higher antibody titer in SL7027 (pVAX-σB-σC) than that in SL7207 (pVAX-σC) group showed that co-expressing σB and σC could improve antibody production. IFN-γ detection showed that significant higher IFN-γ was generated both in groups SL7027 (pVAX-σB-σC) and SL7207 (pVAX-σC). Subsequent challenge showed that SL7207 (pVAX-σB), SL7207 (pVAX-σC) and SL7027 (pVAX-σB-σC) conferred 50%, 75% and 87.5% respectively.
禽呼肠孤病毒（ARV）是家禽业中的一种重要病原体，会造成巨大的经济损失。由于减毒鼠伤寒沙门氏菌已被用作DNA疫苗转移的有效载体，因此在本研究中，我们评估了两种由鼠伤寒沙门氏菌介导的DNA疫苗引发抗体产生的能力。用SL7207（pVAX-σB）、SL7207（pVAX-σC）和SL7027（pVAX-σB-σC）分别对SPF鸡进行三次免疫。结果表明，抗体产生高度依赖于免疫次数，血清抗体IgG和小肠黏膜抗体IgA的可检测抗体在第4周产生，并在第6周进一步提高，SL7207（pVAX-σC）组的抗体滴度高于SL7207（pVAX-σB）组，表明SL7207（pVAX-σC）在产生抗体方面比SL7207（pVAX-σB）更有效。SL7027（pVAX-σB-σC）组的抗体滴度高于SL7207（pVAX-σC）组，表明共表达σB和σC可提高抗体产生。IFN-γ检测表明，SL7027（pVAX-σB-σC）组和SL7207（pVAX-σC）组均产生了显著更高的IFN-γ。随后的攻毒试验表明，SL7207（pVAX-σB）、SL7207（pVAX-σC）和SL7027（pVAX-σB-σC）分别提供了50%、75%和87.5%的保护率。
